Development of a luminex-based assay for the detection of anti-capripoxvirus and rift valley fever virus antibodies in domestic ruminants.

IF 4 3区医学 Q2 VIROLOGY Virology Journal Pub Date : 2024-12-27 DOI:10.1186/s12985-024-02602-9

Francisco J Berguido, Tirumala Bharani Kumar Settypalli, Curé Georges Tshilenge Mbuyi, Mame Thierno Bakhom, Petrus Jansen van Vuren, Janusz T Pawęska, Giovanni Cattoli, Reingard Grabherr, Charles Euloge Lamien

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Abstract

The three members of the genus capripoxvirus (CaPV), lumpy skin disease virus (LSDV), sheeppox virus (SPPV), and goatpox virus (GTPV) have common hosts and areas of overlapping geographical distribution with Rift Valley fever virus (RVFV). Hence, to ensure more cost-effective disease surveillance we developed and evaluated a Luminex assay for the simultaneous detection of antibodies against CaPV and RVFV in domestic ruminants. In cattle, the assay had a sensitivity (Se) of 98.7% and a specificity (Sp) of 98.3% in detecting anti-LSDV antibodies; both diagnostic parameters were 100% for the detection of anti-RVFV antibodies in this species. In sheep and goats, Se and Sp were 100% for the detection of anti-SPPV and anti-GTPV antibodies while they were 100% and 98.9%, respectively for the detection of anti-RVFV antibody. The assay did not cross react with anti-parapoxvirus antibodies of cattle, sheep, and goats. This multiplex serological assay offers a practical tool for accurate detection and monitoring of the immunological status of domestic ruminant populations against veterinary and socio-economically important capripox- and phleboviral infections, thus has the potential to aid in the strategic application of vaccination programmes.

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家畜反刍动物抗猪痘病毒和裂谷热病毒抗体荧光检测方法的建立。

capripoxvirus （CaPV）属的三个成员，即肿块性皮肤病病毒（LSDV）、羊痘病毒（SPPV）和山羊痘病毒（GTPV）具有共同的宿主和与裂谷热病毒（RVFV）重叠的地理分布区域。因此，为了确保更具成本效益的疾病监测，我们开发并评估了Luminex检测方法，用于同时检测家畜反刍动物的CaPV和RVFV抗体。在牛中，检测抗lsdv抗体的灵敏度（Se）为98.7%，特异性（Sp）为98.3%；这两项诊断参数对该物种的抗裂谷热病毒抗体检测均为100%。在绵羊和山羊中，Se和Sp对抗sppv和抗gtpv抗体的检出率为100%，对抗rvfv抗体的检出率分别为100%和98.9%。该试验与牛、绵羊和山羊的抗副痘病毒抗体无交叉反应。这种多重血清学检测提供了一种实用的工具，可准确检测和监测国内反刍动物种群对兽医和社会经济上重要的capripox和静脉病毒感染的免疫状况，因此有可能有助于疫苗接种规划的战略性应用。

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来源期刊

Virology Journal 医学-病毒学

CiteScore

7.40

自引率

2.10%

发文量

186

审稿时长

1 months

期刊介绍： Virology Journal is an open access, peer reviewed journal that considers articles on all aspects of virology, including research on the viruses of animals, plants and microbes. The journal welcomes basic research as well as pre-clinical and clinical studies of novel diagnostic tools, vaccines and anti-viral therapies. The Editorial policy of Virology Journal is to publish all research which is assessed by peer reviewers to be a coherent and sound addition to the scientific literature, and puts less emphasis on interest levels or perceived impact.