Pathogenicity and antigenic characterization of a novel highly virulent lineage 3 porcine reproductive and respiratory syndrome virus 2.

IF 4.5 2区医学 Q2 IMMUNOLOGY Journal of Microbiology Immunology and Infection Pub Date : 2024-12-21 DOI:10.1016/j.jmii.2024.12.003

Yon-Yip Chan, Cheng-Yao Yang, Chuen-Fu Lin, Sheng-Yuan Wang, Wei-Hao Lin, Ming-Tang Chiou, Chao-Nan Lin

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Abstract

Background/purpose: Porcine reproductive and respiratory syndrome virus (PRRSV) is a pathogen with a negative economic impact on the global swine industry. In 2019, a suspected highly pathogenic strain, NPUST-108-929/2019 (108-929), was isolated from a pig farm in Pingtung with an outbreak of high mortality and analyzed. The characteristics of PRRSV 108-929 have barely been studied.

Methods: This study was to evaluate pathogenicity through animal challenge experiments using PRRSV 108-929 and antigenic characterization of this novel PRRSV.

Results: This PRRSV strain is PRRSV 2, belonging to lineage 3 based on open reading frame 5 sequence analysis. Four putative N-linked glycosylation sites (N32, N35, N44 and N51) are located on glycoprotein 5. Experimental results revealed that high fever occurred at 3 days postinoculation (dpi) in the high-titer inoculation (HIN) group (2 × 10⁴ TCID₅₀/mL), 8 dpi in the high-titer contact (HC) group, 4 dpi in the low-titer inoculation (LIN) group (2 × 10³ TCID₅₀/mL) and 9 dpi in the low-titer contact (LC) group. All pigs in each PRRSV 108-929 challenge and contact group showed severe clinical signs, such as high fever (>40.5 °C) and significant weight loss. Deaths occurred only in the HIN group; the survival rate was 60 %. All the piglets except the control group piglets showed high viremia titers (6.04-8.28 log¹⁰ copies/μL).

Conclusion: The pathogenic characteristics of PRRSV 108-929 suggest that it is a highly virulent PRRSV strain at both the farm and laboratory levels.

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猪繁殖与呼吸综合征新毒株的致病性和抗原特性

背景/目的：猪繁殖与呼吸综合征病毒（PRRSV）是一种对全球养猪业造成负面经济影响的病原体。2019年，在屏东某猪场分离到疑似高致病性菌株npost -108-929/2019(108-929)，并对其进行了分析。PRRSV 108-929的特性研究很少。方法：通过动物攻毒实验对PRRSV 108-929进行致病性评价，并对该新型PRRSV进行抗原鉴定。结果：根据开放阅读框5序列分析，该PRRSV菌株为PRRSV 2，属于谱系3。四个假定的n链糖基化位点（N32、N35、N44和N51）位于糖蛋白5上。实验结果显示，高滴度接种（HIN）组（2 × 104 TCID50/mL）接种后3 d出现高热，高滴度接触（HC）组为8 dpi，低滴度接种（LIN）组（2 × 103 TCID50/mL）为4 dpi，低滴度接触（LC）组为9 dpi。每个PRRSV 108-929攻毒组和接触组的所有猪均出现严重的临床症状，如高热（>40.5°C）和显著体重减轻。死亡仅发生在HIN组；生存率为60%。除对照组外，其余仔猪病毒滴度均较高（6.04 ~ 8.28 log10拷贝/μL）。结论：PRRSV 108-929的致病性特点表明该菌株在农场和实验室水平上都是高毒力的PRRSV毒株。

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来源期刊

Journal of Microbiology Immunology and Infection IMMUNOLOGY-INFECTIOUS DISEASES

CiteScore

15.90

自引率

5.40%

发文量

159

审稿时长

67 days

期刊介绍： Journal of Microbiology Immunology and Infection is an open access journal, committed to disseminating information on the latest trends and advances in microbiology, immunology, infectious diseases and parasitology. Article types considered include perspectives, review articles, original articles, brief reports and correspondence. With the aim of promoting effective and accurate scientific information, an expert panel of referees constitutes the backbone of the peer-review process in evaluating the quality and content of manuscripts submitted for publication.