Genome-wide study of the R2R3-MYB gene family and analysis of HhMYB111r-induced salt tolerance in Hibiscus hamabo Sieb. et Zucc

Abstract

Hibiscus hamabo Sieb. et Zucc. (H. hamabo) is a semi-mangrove plant with excellent stress tolerance that plays a crucial role in the ecological restoration of saline and alkaline areas. It is an ideal candidate species for studying the mechanisms involved in stress tolerance. Although the MYB gene family has preliminarily been characterized in H. hamabo, the specific functions and action mechanisms of the R2R3-MYB genes in this species have not fully been elucidated. In this study, 190 R2R3-MYB genes were identified at the genomic level using bioinformatics methods. The genes were divided into 26 subgroups based on their evolutionary relationships and found to be distributed randomly on 46 chromosomes. RNA sequencing data and subsequent real-time quantitative PCR analysis of 12 differentially expressed R2R3-HhMYB genes showed HhMYB111r to be highly expressed under various abiotic stress conditions. Self-activation and subcellular localization results showed that the intact HhMYB111r had strong self-activation activity and located in both the nucleus and cytoplasm. Overexpression in Arabidopsis significantly improved salt tolerance, and silencing HhMYB111r reduced the tolerance of H. hamabo to salt stress, indicating that HhMYB111r positively regulates the salt stress response. In this first analysis of the R2R3-MYB gene family in H. hamabo, we identified a key salt stress response gene, HhMYB111r, enriching the understanding of MYB function and laying a foundation for exploring the abiotic stress response of plants.