UV-Aged Nanoplastics Increase Mercury Toxicity in a Marine Copepod under Multigenerational Exposure: A Carrier Role

Abstract

Aged plastics possess diverse interactive properties with metals compared to pristine ones. However, the role of aging for nanoplastics (NPs) in being a carrier of mercury (Hg), a common marine environmental pollutant, and their combined effects remain unclear. This study investigated the carrier effect of ultraviolet-aged NPs on Hg and the ensuing toxicity in a marine copepod Tigriopus japonicus under a multigenerational scenario. Aged NPs revealed a better carrier role in Hg bioaccumulation than pristine ones, which was increased by 1.61, 1.52, and 1.54 times in F0, F1, and F2, respectively, probably attributed to increased levels of O-containing functional groups and better adsorption for Hg. Consequently, relative to Hg alone, Hg combined with aged NPs (rather than pristine ones) significantly compromised the copepod’s fitness, e.g., the survival rate decreasing by 74.2 and 62.1% in F1 and F2, respectively. This is possibly linked to the most pronounced transcriptomic response under Hg combined with aged NPs, including disturbed cuticle formation, activated antioxidants, and down-regulation of reproductive genes. Overall, our findings emphasize the non-negligible risk of aged NPs as carriers of toxic metals and provide a better understanding about the long-term effects of coexisting NPs and metal pollution on organisms in real marine environments.