Long-term Exposure to Emamectin Benzoate Impairs Reproductive Health in Adult Zebrafish and Alters Neurodevelopment in Their Offspring

Abstract

Emamectin benzoate (EMB) is a widely used insecticide that can accumulate in aquatic environments under specific conditions of high application intensity or improper management, posing potential risks to aquatic organisms and human health. This study aimed to evaluate the reproductive toxicity of long-term EMB exposure in zebrafish (Danio rerio) and neurodevelopmental toxicity in their offspring. Zebrafish embryos were exposed to various concentrations of EMB (0, 0.1, 1, and 10 μg/L) for up to 120 days. The results revealed a significant decrease in reproductive capacity and gonadal tissue damage in the F0 generation zebrafish. Additionally, the increased oxidative stress levels induced by EMB exposure further exacerbated reproductive toxicity. The F1 generation of zebrafish exhibited a high rate of deformities, reduced body length, decreased swim bladder area, and abnormal swimming behavior. Compared to the control group, zebrafish larvae in the 1 and 10 μg/L EMB exposure groups showed a significant reduction in distance travelled of 18.3% and 36.9% and a significant increase in dwell time of 6.1% and 17.1%. Analysis of neurodevelopment and gene expression in the F1 generation revealed that EMB exposure diminished the development of the central nervous system and further aggravated developmental toxicity through pathways such as oxidative stress, inflammatory response, apoptosis. Notably, maternal exposure to EMB exerted a more significant impact on developmental and neurotoxic effects in the offspring. This study demonstrated that long-term EMB exposure causes significant parental reproductive and offspring neurodevelopmental toxicity in aquatic organisms, thus highlighting the importance of environmental risk assessment and pollution control.