{"title":"Effects of DCA gene overexpression in transgenic Dunaliella parva","authors":"Lingru Ruan, Bingfeng Zhou, Yanyan Liang, Xiumin Zhang, Lina Wu, Limei Huang, Shiyu Liang, Changhua Shang","doi":"10.1007/s10482-024-02054-9","DOIUrl":null,"url":null,"abstract":"<div><p>Duplicated carbonic anhydrase was a member of carbonic anhydrase family. The ubiquitous carbonic anhydrase (CA) plays important roles in plants. However, the role of CA in <i>Dunaliella parva</i> remains to be further explored. In this study, a member of the CAs family was isolated from <i>D. parva</i> and named <i>DpDCA</i>. It was transferred into <i>D. parva</i> by genetic engineering techniques. The carotenoid, total carbohydrate, starch, protein, and oil contents of transgenic <i>D. parva</i> increased by 16.31%, 31.68%, 43.97%, 52.91%, and 12.32%, compared to control. Perhaps <i>DpDCA</i> affected photosynthesis through cis-element. Therefore, this study lays a foundation for studying the function of <i>DpDCA</i> gene in <i>D. parva</i>.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"118 2","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10482-024-02054-9","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Duplicated carbonic anhydrase was a member of carbonic anhydrase family. The ubiquitous carbonic anhydrase (CA) plays important roles in plants. However, the role of CA in Dunaliella parva remains to be further explored. In this study, a member of the CAs family was isolated from D. parva and named DpDCA. It was transferred into D. parva by genetic engineering techniques. The carotenoid, total carbohydrate, starch, protein, and oil contents of transgenic D. parva increased by 16.31%, 31.68%, 43.97%, 52.91%, and 12.32%, compared to control. Perhaps DpDCA affected photosynthesis through cis-element. Therefore, this study lays a foundation for studying the function of DpDCA gene in D. parva.
期刊介绍：
Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.
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