Amal Mahmoud Aboelmaaty, Abdalla E A Elgharieb, Hazem Ahmed El-Debaky, Jamal M H Alkhadrawy, Mostafa M Abou-Ahmed, Abdelraouf M Ghallab
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引用次数: 0
Abstract
Background: Senility influences fertility in women and companion animals, especially horses.
Aim: This study aimed to investigate the effect of aging in horses on the daily changes in the dominant follicle (DF) dynamics and hemodynamics, antimüllerian hormone (AMH), enzymes, antioxidants, and ovarian hormones during the estrous cycle.
Methods: Ovaries of old mares (n = 5, age >20 years) and young native mares (n = 6, age <10 years) were scanned during 6 different estrous cycles from March 2022 to August 2023 with Doppler ultrasound. The DF diameter and color area, the corpus luteum (CL) diameter, and the uterine horn area and color area were determined. In collected blood samples with each ultrasound and Doppler scanning, AMH, estradiol (E2), progesterone (P4), cholesterol, myeloperoxidase, catalase, glutathione peroxidase, lactate dehydrogenase (LDH), alkaline phosphatase, and nitric oxide (NO) concentrations were determined.
Results: Age significantly affected the DF area ( p < 0.0001), color area ( p < 0.0001), color area % (p = 0.020), CL area (p = 0.033), uterine horn area ( p < 0.0001), ovarian artery pulsatility index (PI, p < 0.05), E2 (p < 0.001), cholesterol ( p < 0.0001), LDH ( p < 0.0001), and NO ( p < 0.0001). Aging tended (p > 0.05) to decrease the DF antrum diameter but significantly decreased (p < 0.05) its area, antrum area, and color area % in addition to the decrease (p < 0.01) in the uterine horn diameter and area, estradiol, total cholesterol, LDH, NO, aging increased (p < 0.05) CL area and the ovarian artery PI, and tended (p > 0.05) to increase the ovarian artery resistance index.
Conclusion: Aging in mares did not disturb AMH, the ovarian macro-environmental dynamics, follicle growth, and recruiting but the disrupted blood flow mediators, enzymes, and antioxidant status may affect the intrafollicular mediators and influence the oocyte quality.
期刊介绍：
Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.
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