Comprehensive Optimization of Packing Parameters for Hydraulic-Packed Capillary Columns.

IF 1.8 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS Rapid Communications in Mass Spectrometry Pub Date : 2025-04-15 DOI:10.1002/rcm.9966

Chunqiu Hu, Hang Fu, Lulu Wang, Yuan Gao, Ruidong Li, Zhen Li, Shuai Li, Xiaowang Xie, Chongxi Ren, Fang Guo, Minjia Tan, Linhui Zhai

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引用次数: 0

Abstract

Rationale: The performance of the capillary column directly impacts the separation efficiency of complex sample in liquid chromatography-mass spectrometry-based proteomics studies. The hydraulic packing system offers an effective solution by reducing packing time and expediting the preparation process of column preparation. However, its operational complexity and strict parameter regulation requirements hinder efficient application.

Methods: This study developed an innovative device for the hydraulic capillary column packing, followed by a systematic optimization and evaluation of crucial parameters such as packing pressure, magnetic stirring rate, concentration of solid-phase material, and choice of packing solvent in the capillary column packing process.

Results: The comprehensive evaluation of the prepared capillary columns showed that the hydraulic packing method enabled rapid and large-scale preparation, while also demonstrating exceptional stability and reproducibility.

Conclusions: The proposed hydraulic capillary column packing strategy in this study holds theoretical and practical implications for advancing the technology of column preparation, enhancing separation efficiency, and expanding the depth of analysis in proteomics research.

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.