Comprehensive Optimization of Packing Parameters for Hydraulic-Packed Capillary Columns

IF 1.7 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS Rapid Communications in Mass Spectrometry Pub Date : 2024-12-30 DOI:10.1002/rcm.9966

Chunqiu Hu, Hang Fu, Lulu Wang, Yuan Gao, Ruidong Li, Zhen Li, Shuai Li, Xiaowang Xie, Chongxi Ren, Fang Guo, Minjia Tan, Linhui Zhai

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Abstract

Rationale

The performance of the capillary column directly impacts the separation efficiency of complex sample in liquid chromatography–mass spectrometry–based proteomics studies. The hydraulic packing system offers an effective solution by reducing packing time and expediting the preparation process of column preparation. However, its operational complexity and strict parameter regulation requirements hinder efficient application.

Methods

This study developed an innovative device for the hydraulic capillary column packing, followed by a systematic optimization and evaluation of crucial parameters such as packing pressure, magnetic stirring rate, concentration of solid-phase material, and choice of packing solvent in the capillary column packing process.

Results

The comprehensive evaluation of the prepared capillary columns showed that the hydraulic packing method enabled rapid and large-scale preparation, while also demonstrating exceptional stability and reproducibility.

Conclusions

The proposed hydraulic capillary column packing strategy in this study holds theoretical and practical implications for advancing the technology of column preparation, enhancing separation efficiency, and expanding the depth of analysis in proteomics research.

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水力填充毛细管柱填料参数的综合优化。

原理：在基于液相色谱-质谱的蛋白质组学研究中，毛细管柱的性能直接影响复杂样品的分离效率。液压充填系统缩短了充填时间，加快了柱前制备过程，是一种有效的解决方案。然而，其操作的复杂性和严格的参数调节要求阻碍了其高效应用。方法：研制了一种新型液压毛细管柱填料装置，对毛细管柱填料过程中填料压力、磁力搅拌速率、固相物料浓度、填料溶剂选择等关键参数进行了系统优化和评价。结果：对制备的毛细管柱的综合评价表明，液压填料法能够快速、大规模地制备毛细管柱，同时具有良好的稳定性和重复性。结论：本研究提出的液压毛细管柱填充策略对蛋白质组学研究中柱制备技术的进步、分离效率的提高、分析深度的拓展具有理论和实践意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.