{"title":"Isotropic 100-nm resolution live-cell imaging with 4Pi-SIM","authors":"","doi":"10.1038/s41592-024-02514-0","DOIUrl":null,"url":null,"abstract":"We developed 4Pi-SIM, which integrates 4Pi microscopy with structured illumination microscopy to achieve isotropic optical resolution through interference in both illumination and detection. We demonstrate its capabilities by time-lapse volumetric imaging of various subcellular structures at 100-nm resolution.","PeriodicalId":18981,"journal":{"name":"Nature Methods","volume":"22 2","pages":"233-234"},"PeriodicalIF":32.1000,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature Methods","FirstCategoryId":"99","ListUrlMain":"https://www.nature.com/articles/s41592-024-02514-0","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
We developed 4Pi-SIM, which integrates 4Pi microscopy with structured illumination microscopy to achieve isotropic optical resolution through interference in both illumination and detection. We demonstrate its capabilities by time-lapse volumetric imaging of various subcellular structures at 100-nm resolution.
期刊介绍：
Nature Methods is a monthly journal that focuses on publishing innovative methods and substantial enhancements to fundamental life sciences research techniques. Geared towards a diverse, interdisciplinary readership of researchers in academia and industry engaged in laboratory work, the journal offers new tools for research and emphasizes the immediate practical significance of the featured work. It publishes primary research papers and reviews recent technical and methodological advancements, with a particular interest in primary methods papers relevant to the biological and biomedical sciences. This includes methods rooted in chemistry with practical applications for studying biological problems.
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