Wolbachia pipientis Modulates Germline Stem Cells and Gene Expression Associated with Ubiquitination and Histone Lysine Trimethylation to Rescue Fertility Defects in Drosophila.

IF 3.3 3区生物学 Q2 GENETICS & HEREDITY Genetics Pub Date : 2024-12-31 DOI:10.1093/genetics/iyae220

Catherine H Kagemann, Jaclyn E Bubnell, Gabriela M Colocho, Daniela Arana, Charles F Aquadro

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Abstract

Wolbachia pipientis are maternally transmitted endosymbiotic bacteria commonly found in arthropods and nematodes. These bacteria manipulate reproduction of the host to increase their transmission using mechanisms, such as cytoplasmic incompatibility, that favor infected female offspring. The underlying mechanisms of reproductive manipulation by W. pipientis remain unresolved. Interestingly, W. pipientis infection partially rescues female fertility in flies containing hypomorphic mutations of bag of marbles (bam) in D. melanogaster, which plays a key role in germline stem cell (GSC) daughter differentiation. Using RNA-seq, we find W. pipientis infection in bam hypomorphic females results in differential expression of many of bam's genetic and physical interactors and enrichment of ubiquitination and histone lysine methylation genes. We find that W. pipientis also rescues the fertility and GSC functions of a subset of these genes when knocked down with RNAi in a wildtype bam genotype. Our results show that W. pipientis interacts with ubiquitination and histone lysine methylation genes which could be integral to the mechanism by which W. pipientis modulates GSC gene function.

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调节生殖系干细胞和泛素化和组蛋白赖氨酸三甲基化相关的基因表达以挽救果蝇的生育缺陷。

沃尔巴克氏体是一种母体传播的内共生细菌，常见于节肢动物和线虫。这些细菌利用细胞质不相容等有利于受感染的雌性后代的机制操纵宿主的繁殖以增加其传播。pipientis的生殖操纵的潜在机制尚不清楚。有趣的是，pipientis感染部分地挽救了含有黑腹金蝇（D. melanogaster）弹袋（bag of marbles, bam）亚形态突变的果蝇的雌性生育能力，该突变在种系干细胞（GSC）子细胞分化中起关键作用。利用RNA-seq技术，我们发现在bam的半胚雌性中，pipientis感染导致bam的许多遗传和物理相互作用因子的差异表达以及泛素化和组蛋白赖氨酸甲基化基因的富集。我们发现，当在野生型bam基因型中被RNAi敲除时，pipientis也可以挽救这些基因的一个子集的生育能力和GSC功能。我们的研究结果表明，pipientis与泛素化和组蛋白赖氨酸甲基化基因相互作用，这可能是pipientis调节GSC基因功能的机制的一部分。

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来源期刊

Genetics GENETICS & HEREDITY-

CiteScore

6.90

自引率

6.10%

发文量

177

审稿时长

1.5 months

期刊介绍： GENETICS is published by the Genetics Society of America, a scholarly society that seeks to deepen our understanding of the living world by advancing our understanding of genetics. Since 1916, GENETICS has published high-quality, original research presenting novel findings bearing on genetics and genomics. The journal publishes empirical studies of organisms ranging from microbes to humans, as well as theoretical work. While it has an illustrious history, GENETICS has changed along with the communities it serves: it is not your mentor''s journal. The editors make decisions quickly – in around 30 days – without sacrificing the excellence and scholarship for which the journal has long been known. GENETICS is a peer reviewed, peer-edited journal, with an international reach and increasing visibility and impact. All editorial decisions are made through collaboration of at least two editors who are practicing scientists. GENETICS is constantly innovating: expanded types of content include Reviews, Commentary (current issues of interest to geneticists), Perspectives (historical), Primers (to introduce primary literature into the classroom), Toolbox Reviews, plus YeastBook, FlyBook, and WormBook (coming spring 2016). For particularly time-sensitive results, we publish Communications. As part of our mission to serve our communities, we''ve published thematic collections, including Genomic Selection, Multiparental Populations, Mouse Collaborative Cross, and the Genetics of Sex.