{"title":"Genome-wide analysis of <i>Nicotiana tabacum</i> IDD genes identifies <i>NtIDD9</i> as a regulator of leaf angle.","authors":"Zefeng Li, Peijian Cao, Huabing Liu, Jianfeng Zhang, Zhaopeng Luo, Hui Zhang, Mingzhu Wu, Xiaodong Xie","doi":"10.3389/fpls.2024.1496351","DOIUrl":null,"url":null,"abstract":"<p><p>The INDETERMINATE DOMAIN (IDD) gene family, encoding a class of C2H2 transcription factor, played diverse roles in land plants. The IDD family in tobacco (<i>Nicotiana tabacum</i>) has not been characterized. In this study, 26 NtIDDs were identified in the tobacco genome. Phylogenetic analysis showed that NtIDDs were divided into five groups. Motif analysis revealed that the ID domain was conserved in NtIDDs. Gene duplication analysis demonstrated that segmental/whole-genome duplication and dispersed duplication would have occurred in NtIDDs. <i>Cis-</i>element analysis predicted that hormone-, stress-, and development-related elements are located in NtIDD promoters. Expression analysis revealed tissue preference patterns and differential hormone responses in NtIDDs. Further investigations on the function of <i>NtIDD9</i> exhibited increased leaf angle degrees in RNA silencing plants. Cellular localization suggested that <i>NtIDD9</i> expressed in the endodermis of the leaf petiole base. Subcellular localization analysis revealed that the NtIDD9 protein was located in the nucleus. Hormone quantification found that the levels of auxin, ABA, JA, and GA were significantly changed in <i>NtIDD9</i>-silenced plants. Thus, the study suggested that <i>NtIDD9</i> played a crucial role in modulation of leaf angle development. Overall, these findings lay foundations for future function and mechanism research on IDDs in tobacco.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"15 ","pages":"1496351"},"PeriodicalIF":4.1000,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11693677/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Plant Science","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3389/fpls.2024.1496351","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
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Abstract
The INDETERMINATE DOMAIN (IDD) gene family, encoding a class of C2H2 transcription factor, played diverse roles in land plants. The IDD family in tobacco (Nicotiana tabacum) has not been characterized. In this study, 26 NtIDDs were identified in the tobacco genome. Phylogenetic analysis showed that NtIDDs were divided into five groups. Motif analysis revealed that the ID domain was conserved in NtIDDs. Gene duplication analysis demonstrated that segmental/whole-genome duplication and dispersed duplication would have occurred in NtIDDs. Cis-element analysis predicted that hormone-, stress-, and development-related elements are located in NtIDD promoters. Expression analysis revealed tissue preference patterns and differential hormone responses in NtIDDs. Further investigations on the function of NtIDD9 exhibited increased leaf angle degrees in RNA silencing plants. Cellular localization suggested that NtIDD9 expressed in the endodermis of the leaf petiole base. Subcellular localization analysis revealed that the NtIDD9 protein was located in the nucleus. Hormone quantification found that the levels of auxin, ABA, JA, and GA were significantly changed in NtIDD9-silenced plants. Thus, the study suggested that NtIDD9 played a crucial role in modulation of leaf angle development. Overall, these findings lay foundations for future function and mechanism research on IDDs in tobacco.
期刊介绍：
In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches.
Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.
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