Development of a ZnNiMOF@CNT-based MIP electrochemical sensor: Toward the selective detection of creatinine in urine and saliva

Abstract

Creatinine is a product of muscle metabolism in the body, it's normally filtered out by the glomeruli. However, creatinine values will deviate from normal when renal function is disturbed. Therefore, it is of great significance to develop an accurate and sensitive creatinine detection method for the preliminary diagnosis of chronic kidney disease(CKD). In this study, Using 1,3, 5-Benzenetricarboxylic acid as ligand, a bimetallic organic framework ZnNiMOF with large specific surface area was prepared, and composite them with carbon nanotubes (CNT). The ratio of the two metals in the bimetal and the ratio of ZnNiMOF to CNT were also optimized. Finally, the optimal proportion was used as the electrode material for molecular imprinting. A molecularly imprinted electrochemical sensor for the accurate and sensitive detection of creatinine was obtained. The obtained sensor has good sensitivity, detection limit of 0.009 μM, and wide detection range (0.03–400 μM). It also shows excellent performance in the detection of actual urine and sweat samples. This study on the molecular imprinted electrochemical sensor provides a new strategy for the subsequent detection of creatinine.