You Wu, Hanbin Wang, Liang Zhang, Wei Zeng, Yongzhen Peng
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引用次数: 0
Abstract
Hydroxylamine (HA) dosing is an effective strategy for promoting partial nitrification (PN); however, its impact on endogenous denitrification remains underexplored. In this study, long-term continuous HA dosing (1.4 mg/L) was introduced for over 110 days in a pilot-scale anaerobic/aerobic/anoxic (AOA) system treating municipal wastewater (66.7-75 m3/d). The HA dosing significantly increased the nitrite accumulation ratio to 67.6 ± 5.0 % (p<0.001) and reduced the effluent total inorganic nitrogen concentration from 6.2 ± 2.0 to 2.4 ± 1.1 mg/L (p<0.001), achieving a nitrogen removal efficiency of 87.4 ± 4.5% (p<0.001) at a hydraulic retention time of 8 hours. During the HA dosing, aerobic nitrogen removal contribution increased from 2.4 ± 3.4 % to 25.8 ± 8.1 % (p<0.001), and the anoxic nitrogen removal rate improved from 1.63 ± 0.11 to 2.35 ± 0.13 mg N/(L·h) (p<0.001). Enhanced nitrogen removal was not only achieved through the rapid establishment of PN but also driven by the long-term impact of HA dosing on microbial community dynamics. Multi-omics analyses revealed that HA disrupted the polyphosphate (poly-P) cycle, evidenced by enhanced transcription of ppx (poly-P degradation) and suppressed ppk (poly-P synthesis), thereby reducing energy availability for phosphate-accumulating organisms (PAOs) and shifting the carbon source competition toward glycogen-accumulating organisms (GAOs), with Ca. Competibacter abundance increased from 0.16% to 1.13% (p < 0.001). The economic analysis demonstrated that HA reduced sludge production by 11.2% and saved operating costs by 31.4–42.8% compared to conventional carbon sources. These findings highlight the potential of HA dosing to achieve sustainable and highly efficient wastewater treatment.
期刊介绍:
Water Research, along with its open access companion journal Water Research X, serves as a platform for publishing original research papers covering various aspects of the science and technology related to the anthropogenic water cycle, water quality, and its management worldwide. The audience targeted by the journal comprises biologists, chemical engineers, chemists, civil engineers, environmental engineers, limnologists, and microbiologists. The scope of the journal include:
•Treatment processes for water and wastewaters (municipal, agricultural, industrial, and on-site treatment), including resource recovery and residuals management;
•Urban hydrology including sewer systems, stormwater management, and green infrastructure;
•Drinking water treatment and distribution;
•Potable and non-potable water reuse;
•Sanitation, public health, and risk assessment;
•Anaerobic digestion, solid and hazardous waste management, including source characterization and the effects and control of leachates and gaseous emissions;
•Contaminants (chemical, microbial, anthropogenic particles such as nanoparticles or microplastics) and related water quality sensing, monitoring, fate, and assessment;
•Anthropogenic impacts on inland, tidal, coastal and urban waters, focusing on surface and ground waters, and point and non-point sources of pollution;
•Environmental restoration, linked to surface water, groundwater and groundwater remediation;
•Analysis of the interfaces between sediments and water, and between water and atmosphere, focusing specifically on anthropogenic impacts;
•Mathematical modelling, systems analysis, machine learning, and beneficial use of big data related to the anthropogenic water cycle;
•Socio-economic, policy, and regulations studies.