Interaction of AURKA with TRIM28 revives dormant LSCC cells via Akt signaling pathway to promote LSCC metastasis.

IF 5.3 2区医学 Q1 ONCOLOGY Cancer Cell International Pub Date : 2025-01-03 DOI:10.1186/s12935-024-03620-x

Liyun Yang, Liang Geng, Xinxin Zhang, Jing Lu, Hao Zhang, Geping Wu, Shuixian Huang

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Abstract

Background: Specific molecular mechanisms by which AURKA promoted LSCC metastasis were still unknown.

Methods: Bioinformatic analysis was performed the relationship between TRIM28 and LSCC. Immunohistochemistry, Co-IP assay, Rt-PCR and Western Blot were used to examine the expression of related molecular. Flow cytometry was used to examine cell numbers of G0/G1 phase. Plate colony formation, wound healing, migration, invasion and tail vein injection in nude mice assays were applied to examine the proliferation, movement, migration, invasion and metastasis of LSCC.

Results: TRIM28 was significantly correlated with LSCC. TRIM28 highly expressed in LSCC and the high TRIM28 expression was related to TNM stage and poor clinical prognosis. Furthermore, AURKA could regulate TRIM28. In addition, deprivation TRIM28 expression induced LSCC cells into dormant state and inhibited LSCC metastasis. Akt signaling pathway played an essential role in promoting the tumor-promoting effects induced by TRIM28.

Conclusion: AURKA mediated TRIM28 to revive dormant LSCC cells via Akt signaling pathway to promote LSCC metastasis, targeting TRIM28 might provide a potential treatment strategy for LSCC.

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AURKA与TRIM28通过Akt信号通路激活休眠LSCC细胞，促进LSCC转移。

背景：AURKA促进LSCC转移的具体分子机制尚不清楚。方法：对TRIM28与LSCC的关系进行生物信息学分析。采用免疫组织化学、Co-IP法、Rt-PCR和Western Blot检测相关分子的表达。流式细胞术检测G0/G1期细胞数量。采用裸鼠平板集落形成、创面愈合、迁移、侵袭及尾静脉注射等方法观察LSCC的增殖、移动、迁移、侵袭和转移。结果：TRIM28与LSCC有显著相关性。TRIM28在LSCC中高表达，且与TNM分期及临床预后差有关。此外，AURKA还能调控TRIM28。此外，剥夺TRIM28表达可诱导LSCC细胞进入休眠状态，抑制LSCC转移。Akt信号通路在促进TRIM28诱导的促肿瘤作用中发挥了重要作用。结论：AURKA介导的TRIM28通过Akt信号通路激活休眠LSCC细胞，促进LSCC转移，靶向TRIM28可能是治疗LSCC的一种潜在策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer Cell International ONCOLOGY-

CiteScore

10.90

自引率

1.70%

发文量

360

审稿时长

1 months

期刊介绍： Cancer Cell International publishes articles on all aspects of cancer cell biology, originating largely from, but not limited to, work using cell culture techniques. The journal focuses on novel cancer studies reporting data from biological experiments performed on cells grown in vitro, in two- or three-dimensional systems, and/or in vivo (animal experiments). These types of experiments have provided crucial data in many fields, from cell proliferation and transformation, to epithelial-mesenchymal interaction, to apoptosis, and host immune response to tumors. Cancer Cell International also considers articles that focus on novel technologies or novel pathways in molecular analysis and on epidemiological studies that may affect patient care, as well as articles reporting translational cancer research studies where in vitro discoveries are bridged to the clinic. As such, the journal is interested in laboratory and animal studies reporting on novel biomarkers of tumor progression and response to therapy and on their applicability to human cancers.