Isolation and characterization of ɸEcM-vB1 bacteriophage targeting multidrug-resistant Escherichia coli.

IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES BMC Research Notes Pub Date : 2025-01-03 DOI:10.1186/s13104-024-07033-x

Rania Abozahra, Dina Shlkamy, Sarah M Abdelhamid

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Abstract

Objectives: The aim of this study is to screen for, isolate and characterize a bacteriophage designated ɸEcM-vB1 with confirmed lytic activity against multidrug-resistant (MDR) E. coli. Methods done in this research are bacteriophage isolation, purification, titer determination, bacteriophage morphology, host range determination, bacteriophage latent period and burst size determination, genomic analysis by restriction enzymes, and bacteriophage total protein content determination.

Results: ɸEcM-vB1 bacteriophage exhibited high lytic activity against different MDR E. coli isolates and showed stability over wide pH and temperature range. It belongs to the Myoviridae family of the caudovirales order according to TEM. It had a latent period of 5 min and an average burst size of 271.72 pfu/cell. Genomic analysis revealed that it is susceptible to digestion by EcoRI. Ten structural proteins were detected by SDS-PAGE. ɸEcM-vB1 is considered a promising candidate for phage therapy applications.

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靶向多重耐药大肠杆菌的噬菌体的分离与鉴定

目的：筛选、分离并鉴定一株具有抗多药耐药（MDR）大肠杆菌裂解活性的噬菌体。本研究采用噬菌体分离、纯化、效价测定、噬菌体形态、宿主范围测定、噬菌体潜伏期和爆发大小测定、限制性内切酶基因组分析、噬菌体总蛋白含量测定等方法。结果：h EcM-vB1噬菌体对不同的耐多药大肠杆菌菌株具有较高的裂解活性，并在较宽的pH和温度范围内表现出稳定性。根据TEM，它属于尾状病毒目的肌病毒科。潜伏期为5 min，平均爆发量为271.72 pfu/细胞。基因组分析显示其易被EcoRI消化。SDS-PAGE检测到10个结构蛋白。EcM-vB1被认为是噬菌体治疗应用的有前途的候选者。

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来源期刊

BMC Research Notes Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

3.60

自引率

0.00%

发文量

363

审稿时长

15 weeks

期刊介绍： BMC Research Notes publishes scientifically valid research outputs that cannot be considered as full research or methodology articles. We support the research community across all scientific and clinical disciplines by providing an open access forum for sharing data and useful information; this includes, but is not limited to, updates to previous work, additions to established methods, short publications, null results, research proposals and data management plans.