Application of an efficient adenine base editing system in developing Danshen (Salvia miltiorrhiza) dinitroaniline-resistant germplasm

IF 5.7 1区 农林科学 Q1 HORTICULTURE Horticultural Plant Journal Pub Date : 2024-12-30 DOI:10.1016/j.hpj.2024.08.007
Huanan Han, Ziwen Wu, Zaijie Zhang, Yusen Qu, Yingcan Wang, Pingping Wang, Changle Ma
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Abstract

Chinese herbal medicine Danshen (Salvia miltiorrhiza) has suggested therapeutic effects on cardiovascular and cerebrovascular diseases, inflammatory reactions and tumors, perhaps due to secondary metabolites, such as tanshinone. Danshen is sensitive to most broad-spectrum herbicides, thus it is a challenge to manage weeds during planting. Although CRISPR/Cas gene editing has been used to knock out important genes in Danshen, the more accurate base editing method has rarely been applied to this species. We developed an efficient adenine base editing system for Danshen and observed its editing effect in callus and transgenic plants. To demonstrate the efficacy of this technique, we generated several novel Danshen germplasms with mutant α-tubulins, some of which displayed resistance to a dinitroaniline herbicide, pendimethalin, in Danshen planting. We also found distinct effects of the different Danshen mutant tubulin homologues on dinitroaniline sensitivity, perhaps indicating a functional differentiation of the α-tubulin genes. Our work provides a new gene modification method for Danshen breeding, which might be extended to other medicinal plants.
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高效腺嘌呤碱基编辑系统在丹参二硝基苯胺抗性种质开发中的应用
中药丹参(丹参)被认为对心脑血管疾病、炎症反应和肿瘤有治疗作用,可能是由于其次生代谢产物,如丹参酮。丹参对大多数广谱除草剂敏感,因此在种植过程中对杂草进行治理是一个挑战。虽然CRISPR/Cas基因编辑已经被用于敲除丹参的重要基因,但更精确的碱基编辑方法很少应用于该物种。我们开发了一种高效的丹参腺嘌呤碱基编辑系统,并观察了其在愈伤组织和转基因植株上的编辑效果。为了证明该技术的有效性,我们在丹参种植中获得了几种具有α-微管蛋白突变体的新型丹参种质,其中一些对二硝基苯胺类除草剂戊二甲基灵具有抗性。我们还发现不同丹参突变体微管蛋白同源物对二硝基苯胺敏感性的不同影响,这可能表明α-微管蛋白基因的功能分化。本研究为丹参育种提供了一种新的基因修饰方法,可推广到其他药用植物中。
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索莱宝
cryptotanshinone
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agar
来源期刊
Horticultural Plant Journal
Horticultural Plant Journal Environmental Science-Ecology
CiteScore
9.60
自引率
14.00%
发文量
293
审稿时长
33 weeks
期刊介绍: Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.
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