{"title":"Application of an efficient adenine base editing system in developing Danshen (Salvia miltiorrhiza) dinitroaniline-resistant germplasm","authors":"Huanan Han, Ziwen Wu, Zaijie Zhang, Yusen Qu, Yingcan Wang, Pingping Wang, Changle Ma","doi":"10.1016/j.hpj.2024.08.007","DOIUrl":null,"url":null,"abstract":"Chinese herbal medicine Danshen (<ce:italic>Salvia miltiorrhiza</ce:italic>) has suggested therapeutic effects on cardiovascular and cerebrovascular diseases, inflammatory reactions and tumors, perhaps due to secondary metabolites, such as tanshinone. Danshen is sensitive to most broad-spectrum herbicides, thus it is a challenge to manage weeds during planting. Although CRISPR/Cas gene editing has been used to knock out important genes in Danshen, the more accurate base editing method has rarely been applied to this species. We developed an efficient adenine base editing system for Danshen and observed its editing effect in callus and transgenic plants. To demonstrate the efficacy of this technique, we generated several novel Danshen germplasms with mutant α-tubulins, some of which displayed resistance to a dinitroaniline herbicide, pendimethalin, in Danshen planting. We also found distinct effects of the different Danshen mutant tubulin homologues on dinitroaniline sensitivity, perhaps indicating a functional differentiation of the α-tubulin genes. Our work provides a new gene modification method for Danshen breeding, which might be extended to other medicinal plants.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"409 1","pages":""},"PeriodicalIF":5.7000,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Horticultural Plant Journal","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.hpj.2024.08.007","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"HORTICULTURE","Score":null,"Total":0}
引用次数: 0
Abstract
Chinese herbal medicine Danshen (Salvia miltiorrhiza) has suggested therapeutic effects on cardiovascular and cerebrovascular diseases, inflammatory reactions and tumors, perhaps due to secondary metabolites, such as tanshinone. Danshen is sensitive to most broad-spectrum herbicides, thus it is a challenge to manage weeds during planting. Although CRISPR/Cas gene editing has been used to knock out important genes in Danshen, the more accurate base editing method has rarely been applied to this species. We developed an efficient adenine base editing system for Danshen and observed its editing effect in callus and transgenic plants. To demonstrate the efficacy of this technique, we generated several novel Danshen germplasms with mutant α-tubulins, some of which displayed resistance to a dinitroaniline herbicide, pendimethalin, in Danshen planting. We also found distinct effects of the different Danshen mutant tubulin homologues on dinitroaniline sensitivity, perhaps indicating a functional differentiation of the α-tubulin genes. Our work provides a new gene modification method for Danshen breeding, which might be extended to other medicinal plants.
期刊介绍:
Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.