Sensitive and selective colorimetric detection of thiophanate-methyl based on a novel Ru-Fe3O4 nanozyme with enhanced peroxidase-like activity

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-01-10 DOI:10.1007/s00604-024-06907-y

Yiwen Gu, Huixiang Yan, Tianwen Bai, Daoyuan Rao, Yiwen Yang, Hongyu Hu, Lei Li, Longhua Guo, Yanbo Zeng

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Abstract

A novel Ru-Fe₃O₄ nanozyme with enhanced peroxidase-like (POD-like) activity was synthesized through a hydrothermal method. Ru-Fe₃O₄ nanozyme was effectively utilized for the detection of thiophanate-methyl (TM) using a colorimetric technique. The POD-like activity of Ru-Fe₃O₄ was found to be superior compared to Fe₃O₄, Rh-Fe₃O₄, and Pd-Fe₃O₄. Ru-Fe₃O₄ provided excellent POD-like activity with Michaelis constants of 0.00645 mM and 0.66714 mM for substrates of 3,3’,5,5’-tetramethylbenzidine and H₂O₂, respectively. The density functional theory calculation showed that Ru-Fe₃O₄ had better H₂O₂ decomposition reactivity compared to Fe₃O₄. Compared with Fe₃O₄, the adsorbed H₂O₂ molecules underwent decomposition more readily on the Ru-Fe₃O₄ catalytic surface facilitating the occurrence of the H₂O₂ catalytic reaction, further suggesting the excellent POD-like activity of Ru-Fe₃O₄. The coordination and electrostatic interactions of Ru-Fe₃O₄ and TM promoted their binding, contributing to TM covering Ru-Fe₃O₄ catalytic site and inhibiting the POD-like activity of Ru-Fe₃O₄. As a result, the sensitive and selective detection of TM using Ru-Fe₃O₄ by a colorimetric method was achieved. The Ru-Fe₃O₄ nanozyme displayed a good linear correlation, with a linear detection range and a detection limit of 0.1–100 and 0.03 μg/mL, respectively. The Ru-Fe₃O₄ nanozyme was used for the determination of TM in soil and water samples with success.

Graphical Abstract

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.