Sensitive and selective colorimetric detection of thiophanate-methyl based on a novel Ru-Fe3O4 nanozyme with enhanced peroxidase-like activity

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-01-10 DOI:10.1007/s00604-024-06907-y

Yiwen Gu, Huixiang Yan, Tianwen Bai, Daoyuan Rao, Yiwen Yang, Hongyu Hu, Lei Li, Longhua Guo, Yanbo Zeng

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Abstract

A novel Ru-Fe₃O₄ nanozyme with enhanced peroxidase-like (POD-like) activity was synthesized through a hydrothermal method. Ru-Fe₃O₄ nanozyme was effectively utilized for the detection of thiophanate-methyl (TM) using a colorimetric technique. The POD-like activity of Ru-Fe₃O₄ was found to be superior compared to Fe₃O₄, Rh-Fe₃O₄, and Pd-Fe₃O₄. Ru-Fe₃O₄ provided excellent POD-like activity with Michaelis constants of 0.00645 mM and 0.66714 mM for substrates of 3,3’,5,5’-tetramethylbenzidine and H₂O₂, respectively. The density functional theory calculation showed that Ru-Fe₃O₄ had better H₂O₂ decomposition reactivity compared to Fe₃O₄. Compared with Fe₃O₄, the adsorbed H₂O₂ molecules underwent decomposition more readily on the Ru-Fe₃O₄ catalytic surface facilitating the occurrence of the H₂O₂ catalytic reaction, further suggesting the excellent POD-like activity of Ru-Fe₃O₄. The coordination and electrostatic interactions of Ru-Fe₃O₄ and TM promoted their binding, contributing to TM covering Ru-Fe₃O₄ catalytic site and inhibiting the POD-like activity of Ru-Fe₃O₄. As a result, the sensitive and selective detection of TM using Ru-Fe₃O₄ by a colorimetric method was achieved. The Ru-Fe₃O₄ nanozyme displayed a good linear correlation, with a linear detection range and a detection limit of 0.1–100 and 0.03 μg/mL, respectively. The Ru-Fe₃O₄ nanozyme was used for the determination of TM in soil and water samples with success.

Graphical Abstract

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基于增强过氧化物酶样活性的新型Ru-Fe3O4纳米酶灵敏选择性比色法检测甲基硫代盐

采用水热法合成了一种具有较强过氧化物酶样活性的Ru-Fe3O4纳米酶。利用Ru-Fe3O4纳米酶对甲基硫代盐（TM）进行比色检测。与Fe3O4、Rh-Fe3O4和Pd-Fe3O4相比，Ru-Fe3O4的pod样活性更强。Ru-Fe3O4对3,3 ',5,5 ' -四甲基联苯胺和H2O2底物具有良好的pod样活性，Michaelis常数分别为0.00645 mM和0.66714 mM。密度泛函理论计算表明，与Fe3O4相比，Ru-Fe3O4具有更好的H2O2分解活性。与Fe3O4相比，吸附的H2O2分子在Ru-Fe3O4催化表面更容易分解，有利于H2O2催化反应的发生，进一步说明Ru-Fe3O4具有优异的类pod活性。Ru-Fe3O4与TM的配位和静电相互作用促进了它们的结合，TM覆盖了Ru-Fe3O4的催化位点，抑制了Ru-Fe3O4的pod样活性。结果表明，采用Ru-Fe3O4比色法对TM进行了灵敏、选择性的检测。钌- fe3o4纳米酶具有良好的线性相关性，线性检测范围为0.1 ~ 100 μg/mL，检出限为0.03 μg/mL。采用Ru-Fe3O4纳米酶测定土壤和水样中的TM，取得了成功。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.