Recordings on PIEZO1-Overexpressing Oocytes in Microgravity

IF 1.3 4区工程技术 Q2 ENGINEERING, AEROSPACE Microgravity Science and Technology Pub Date : 2025-01-06 DOI:10.1007/s12217-024-10155-3

Simon L. Wuest, Geraldine Cerretti, Jennifer Polzer, Simon Gerig, Christoph Zumbühl, Christian Jost, Lukas Rüfenacht, Robert Eberli, Barbara Krucker-Bösch, Julia Traversari, Melanie Horn, Daniel Invernot Pérez, Christina Giger-Lange, Karin F. Rattenbacher-Kiser, Fabian Ille, Gerhard Székely, Soeren S. Lienkamp, Marcel Egli

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Abstract

Exposure to acute and prolonged microgravity triggers numerous physiological adaptations. To date, the underlying molecular mechanisms are not well understood, and several pathways have been proposed. Among other candidates, specific ion channels are hypothesized to be gravity dependent, but it has not been possible to conclusively demonstrate gravity dependency of specific protein entities. Therefore, we developed a miniaturized two-electrode voltage clamp (TEVC) that allowed electrophysiological experiments on Xenopus laevis oocytes using the GraviTower Bremen Prototype (GTB-Pro). The GTB-Pro is capable of flying experiments on a vertical parabolic trajectory, providing microgravity for a few seconds. As an interesting first candidate, we examined whether the nonselective mechanosensitive ion channel PIEZO1 is gravity dependent. The results showed no difference between PIEZO1-overexpressing and control oocytes under acute microgravity conditions.

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微重力下过表达piezo1的卵母细胞的记录

长期暴露在急性微重力环境下会引发许多生理适应。迄今为止，潜在的分子机制尚未得到很好的理解，并提出了几种途径。在其他候选物质中，假设特定离子通道依赖于重力，但不可能最终证明特定蛋白质实体的重力依赖性。因此，我们开发了一种小型化的双电极电压钳（TEVC），利用gravower不来梅原型（GTB-Pro）对非洲爪蟾卵母细胞进行电生理实验。GTB-Pro能够在垂直抛物线轨道上进行飞行实验，提供几秒钟的微重力。作为一个有趣的第一个候选者，我们研究了非选择性机械敏感离子通道PIEZO1是否依赖于重力。结果显示，急性微重力条件下，piezo1过表达与对照卵母细胞无差异。

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来源期刊

Microgravity Science and Technology 工程技术-工程：宇航

CiteScore

3.50

自引率

44.40%

发文量

期刊介绍： Microgravity Science and Technology – An International Journal for Microgravity and Space Exploration Related Research is a is a peer-reviewed scientific journal concerned with all topics, experimental as well as theoretical, related to research carried out under conditions of altered gravity. Microgravity Science and Technology publishes papers dealing with studies performed on and prepared for platforms that provide real microgravity conditions (such as drop towers, parabolic flights, sounding rockets, reentry capsules and orbiting platforms), and on ground-based facilities aiming to simulate microgravity conditions on earth (such as levitrons, clinostats, random positioning machines, bed rest facilities, and micro-scale or neutral buoyancy facilities) or providing artificial gravity conditions (such as centrifuges). Data from preparatory tests, hardware and instrumentation developments, lessons learnt as well as theoretical gravity-related considerations are welcome. Included science disciplines with gravity-related topics are: − materials science − fluid mechanics − process engineering − physics − chemistry − heat and mass transfer − gravitational biology − radiation biology − exobiology and astrobiology − human physiology