{"title":"Genome-wide analysis of DnaJA proteins in Nicotiana tabacum reveals NtDnaJA3 responses to drought stress","authors":"Qiao Wang, Lijun Meng, Zechao Qu, Huan Su, Jiemeng Tao, Peng Lu, Chenfeng Hua, Jinbang Wang, Jianfeng Zhang, Peijian Cao, Jingjing Jin","doi":"10.1186/s40538-024-00726-2","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>DnaJA proteins, a prominent subfamily of the DnaJ family, function as molecular chaperones that respond to various external stresses. Extensive studies on the DnaJ family have been conducted in plants. However, research on this subfamily in tobacco remains relatively scarce.</p><h3>Results</h3><p>In this study, we identified 24 DnaJA genes in tobacco, and classified them into three individual groups. A comprehensive analysis based on gene structure, motif composition, and evolutionary pattern revealed the divergence of tobacco DnaJA genes. For the evolution of the <i>NtDnaJA</i> genes, purification selection was the major factor. In addition, the potential regulatory network unveiled that <i>NtDnaJA</i>s could be regulated by miRNAs and various transcription factors associated with diverse stress responses. Through expression pattern analysis of public RNA-seq datasets and qRT-PCR experiments, it was observed that many <i>NtDnaJAs</i> displayed tissue-specific expression and might play significant roles in different biotic and abiotic stresses. Additionally, the pivotal role of <i>NtDnaJA3</i> in boosting plant drought resistance was confirmed.</p><h3>Conclusions</h3><p>This study provides important perspectives on the evolution of <i>NtDnaJA</i> genes and their involvement in stress responses, laying the groundwork for future research into the roles of DnaJA regulatory genes in tobacco.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":512,"journal":{"name":"Chemical and Biological Technologies in Agriculture","volume":"12 1","pages":""},"PeriodicalIF":5.2000,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-024-00726-2","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical and Biological Technologies in Agriculture","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1186/s40538-024-00726-2","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Background
DnaJA proteins, a prominent subfamily of the DnaJ family, function as molecular chaperones that respond to various external stresses. Extensive studies on the DnaJ family have been conducted in plants. However, research on this subfamily in tobacco remains relatively scarce.
Results
In this study, we identified 24 DnaJA genes in tobacco, and classified them into three individual groups. A comprehensive analysis based on gene structure, motif composition, and evolutionary pattern revealed the divergence of tobacco DnaJA genes. For the evolution of the NtDnaJA genes, purification selection was the major factor. In addition, the potential regulatory network unveiled that NtDnaJAs could be regulated by miRNAs and various transcription factors associated with diverse stress responses. Through expression pattern analysis of public RNA-seq datasets and qRT-PCR experiments, it was observed that many NtDnaJAs displayed tissue-specific expression and might play significant roles in different biotic and abiotic stresses. Additionally, the pivotal role of NtDnaJA3 in boosting plant drought resistance was confirmed.
Conclusions
This study provides important perspectives on the evolution of NtDnaJA genes and their involvement in stress responses, laying the groundwork for future research into the roles of DnaJA regulatory genes in tobacco.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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