Preparation and Characterization of Papain Loaded Phosphatidyl Choline-PLGA Hybrid Nanoparticles as Novel Drug Delivery Systems

IF 4.7 3区工程技术 Q2 ENGINEERING, ENVIRONMENTAL Journal of Polymers and the Environment Pub Date : 2024-10-09 DOI:10.1007/s10924-024-03374-7

Aarzoo Sinha, S. Rupachandra

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Abstract

Polymeric nanoparticles possess the benefits of biocompatibility and stability, and also enable the controlled release of drugs. The matrix of the nanoparticle highlights the potential for an enhancement in the stability and efficacy of encapsulated therapeutic enzymes. The oral administration of lipid-polymer nanoparticle significantly improves mucus penetration, cellular uptake, and intracellular transport. The objective of this study is to develop a highly effective lipid-polymer nanoparticle system that is capable of absorbing Papain in the intestine. Papain loaded PLGA nanoparticles and Papain loaded PLGA-phosphatidylcholine nanoparticles were identified using FTIR analysis. Drug encapsulation efficiency of Papain loaded PLGA nanoparticles was found to be 49.20% and Papain loaded PLGA-Phosphatidylcholine nanoparticles was 77.5%. The drug loading capacity was found to be 3.75% and 6.84% for the Papain loaded PLGA- nanoparticles and Papain loaded PLGA-Phosphatidylcholine nanoparticles respectively. The antibacterial activity of Papain loaded PLGA-PC nanoparticles was found to be higher as compared to PLGA-PC nanoparticle for Staphylococcus aureus and Escherichia coli respectively. About 98% viability was observed in RAW 264.7 macrophage cells treated with the maximum concentration of 100 µg/ml of Papain-loaded PLGA-PC nanoparticles thereby depicting the biocompatibility property of the nanoparticles.

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载木瓜蛋白酶磷脂酰胆碱-聚乳酸杂化纳米颗粒的制备与表征

高分子纳米颗粒具有良好的生物相容性和稳定性，并能实现药物的控释。纳米颗粒的基质突出了胶囊化治疗酶的稳定性和有效性增强的潜力。口服脂质聚合物纳米颗粒可显著改善黏液渗透、细胞摄取和细胞内运输。本研究的目的是开发一种高效的脂质聚合物纳米颗粒系统，能够在肠道中吸收木瓜蛋白酶。利用FTIR对载木瓜蛋白酶PLGA纳米粒子和载木瓜蛋白酶PLGA-磷脂酰胆碱纳米粒子进行了鉴定。结果表明，载木瓜蛋白酶的PLGA纳米颗粒的包封率为49.20%，载木瓜蛋白酶的PLGA-磷脂酰胆碱纳米颗粒的包封率为77.5%。结果表明，载木瓜蛋白酶的PLGA-纳米颗粒和载木瓜蛋白酶的PLGA-磷脂酰胆碱纳米颗粒的载药量分别为3.75%和6.84%。木瓜蛋白酶负载的PLGA-PC纳米粒对金黄色葡萄球菌和大肠杆菌的抑菌活性分别高于PLGA-PC纳米粒。在最大浓度为100µg/ml的木瓜蛋白酶负载PLGA-PC纳米颗粒处理过的RAW 264.7巨噬细胞中，存活率约为98%，从而描述了纳米颗粒的生物相容性。

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来源期刊

Journal of Polymers and the Environment 工程技术-高分子科学

CiteScore

9.50

自引率

7.50%

发文量

297

审稿时长

9 months

期刊介绍： The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.