Nanobody-Based Lateral Flow Immunoassay for Rapid Antigen Detection of SARS-CoV-2 and MERS-CoV Proteins.

IF 3.9 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS ACS Synthetic Biology Pub Date : 2025-02-21 Epub Date: 2025-01-09 DOI:10.1021/acssynbio.4c00592

Yuli Peng, Yaning Huang, Fabian Kiessling, Dominik Renn, Magnus Rueping

引用次数: 0

Abstract

The COVID-19 pandemic has highlighted the critical need for pathogen detection methods that offer both low detection limits and rapid results. Despite advancements in simplifying and enhancing nucleic acid amplification techniques, immunochemical methods remain the preferred methods for mass testing. These methods eliminate the need for specialized laboratories and highly skilled personnel, making home testing feasible. Here, we developed nanobody-based lateral flow assays (LFAs) for the rapid detection of SARS-CoV-2 and MERS-CoV in single and dual formats as point-of-care diagnostic tools. The developed LFAs are highly sensitive and successfully detected analytes at clinically relevant diagnostic cutoff values. Additionally, our results confirmed that the LFAs have a long shelf life and can be produced cost-effectively and with ease.

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基于纳米体的横向流动免疫法快速检测SARS-CoV-2和MERS-CoV蛋白抗原

2019冠状病毒病大流行凸显了对既能提供低检测限又能提供快速结果的病原体检测方法的迫切需要。尽管在简化和增强核酸扩增技术方面取得了进步，免疫化学方法仍然是大规模检测的首选方法。这些方法消除了对专门实验室和高技能人员的需求，使家庭检测成为可能。在这里，我们开发了基于纳米体的横向流动测定法（LFAs），用于快速检测SARS-CoV-2和MERS-CoV，以单一和双重格式作为即时诊断工具。所开发的LFAs具有高度敏感性，并且能够在临床相关诊断截止值下成功检测分析物。此外，我们的研究结果证实了lfa具有较长的保质期，并且可以成本有效且容易地生产。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Synthetic Biology 生物-

CiteScore

8.00

自引率

10.60%

发文量

380

审稿时长

6-12 weeks

期刊介绍： The journal is particularly interested in studies on the design and synthesis of new genetic circuits and gene products; computational methods in the design of systems; and integrative applied approaches to understanding disease and metabolism. Topics may include, but are not limited to: Design and optimization of genetic systems Genetic circuit design and their principles for their organization into programs Computational methods to aid the design of genetic systems Experimental methods to quantify genetic parts, circuits, and metabolic fluxes Genetic parts libraries: their creation, analysis, and ontological representation Protein engineering including computational design Metabolic engineering and cellular manufacturing, including biomass conversion Natural product access, engineering, and production Creative and innovative applications of cellular programming Medical applications, tissue engineering, and the programming of therapeutic cells Minimal cell design and construction Genomics and genome replacement strategies Viral engineering Automated and robotic assembly platforms for synthetic biology DNA synthesis methodologies Metagenomics and synthetic metagenomic analysis Bioinformatics applied to gene discovery, chemoinformatics, and pathway construction Gene optimization Methods for genome-scale measurements of transcription and metabolomics Systems biology and methods to integrate multiple data sources in vitro and cell-free synthetic biology and molecular programming Nucleic acid engineering.