Nitrogen Assimilation Plays a Role in Balancing the Chloroplastic Glutathione Redox Potential Under High Light Conditions.

Abstract

Nitrate reduction requires reducing equivalents produced by the photosynthetic electron transport chain. Therefore, it has been suggested that nitrate assimilation provides a sink for electrons under high light conditions. We tested this hypothesis by monitoring photosynthetic efficiency and the chloroplastic glutathione redox potential (chl-E_GSH) of plant lines with mutated glutamine synthetase 2 (GS2) and ferredoxin-dependent glutamate synthase 1 (GOGAT1). Mutant lines incorporated significantly less isotopically-labelled nitrate into amino acids than wild-type plants, demonstrating impaired nitrogen assimilation. When nitrate assimilation was compromised, photosystem II (PSII) proved more vulnerable to photodamage. The effect of the nitrate assimilation pathway on the chl- E_GSH was monitored using the chloroplast-targeted roGFP2 biosensor (chl-roGFP2). Remarkably, while oxidation followed by reduction of chl-roGFP2 was detected in WT plants in response to high light, oxidation values were stable in the mutant lines, suggesting that chl-E_GSH relaxation after high light-induced oxidation is achieved by diverting excess electrons to the nitrogen assimilation pathway. Importantly, similar ΦPSII and chl-roGFP2 patterns were observed at elevated CO_2, suggesting that mutant phenotypes are not associated with photorespiration activity. Together, these findings indicate that the nitrogen assimilation pathway serves as a sustainable energy dissipation route, ensuring efficient photosynthetic activity and fine-tuning redox metabolism under light-saturated conditions.