Systematic Characterization of the Chemical Constituents of in Vitro and in Vivo of Astragali Radix-Salvia miltiorrhiza Herb Pair by Ultra-High-Performance-Liquid Chromatography-Orbitrap Mass Spectrometry.
Lexin Shu, Shumin Zhang, Jun Qian, Sitong Liu, Huixin Qiu, Qiduo Zhao, Yanru Deng, Yubo Li
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引用次数: 0
Abstract
Rationale: Astragali radix-Salvia miltiorrhiza (AR-SM) is an herb pair with good therapeutic effects and is widely used. In this study, the in vitro and in vivo components of AR-SM were quickly classified and identified based on UHPLC-orbital mass spectrometry. This provided a basis for clarifying the bioactive substances after compatibility of AR and SM.
Methods: Firstly, the related literature of AR and SM were searched through PubMed and CNKI databases, and the molecular formula, retention time, mass spectrum fragments, and other information of its chemical components were sorted out to establish a component database. Extract, blank plasma and drug-containing plasma were analyzed by UHPLC orbital mass spectrometry technology, and then the data were processed by Xcalibur software. Combined with databases and literature, the in vivo and in vitro components of AR-SM were comprehensively analyzed.
Results: A total of 142 components were identified in the extract of AR-SM. The cleavage patterns of flavonoids, terpenoids, phenolic acids, organic acids, saponins, and phenylpropanoids were summarized. Meanwhile, 89 compounds were tentatively identified in rat plasma, including 73 prototypes and 16 metabolites. The main metabolic pathways included oxidation, reduction, methylation, glucuronidation, and sulfation.
期刊介绍：
Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.
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