A hyperspectral open-source imager (HOSI).

IF 4.4 1区 生物学 Q1 BIOLOGY BMC Biology Pub Date : 2025-01-07 DOI:10.1186/s12915-024-02110-w
Jolyon Troscianko
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Abstract

Background: The spatial and spectral properties of the light environment underpin many aspects of animal behaviour, ecology and evolution, and quantifying this information is crucial in fields ranging from optical physics, agriculture/plant sciences, human psychophysics, food science, architecture and materials sciences. The escalating threat of artificial light at night (ALAN) presents unique challenges for measuring the visual impact of light pollution, requiring measurement at low light levels across the human-visible and ultraviolet ranges, across all viewing angles, and often with high within-scene contrast.

Results: Here, I present a hyperspectral open-source imager (HOSI), an innovative and low-cost solution for collecting full-field hyperspectral data. The system uses a Hamamatsu C12880MA micro spectrometer to take single-point measurements, together with a motorised gimbal for spatial control. The hardware uses off-the-shelf components and 3D printed parts, costing around £350 in total. The system can run directly from a computer or smartphone with a graphical user interface, making it highly portable and user-friendly. The HOSI system can take panoramic hyperspectral images that meet the difficult requirements of ALAN research, sensitive to low light around 0.001 cd.m-2, across 320-880 nm range with spectral resolution of ~ 9 nm (FWHM) and spatial resolution of ~ 2 cycles per degree. The independent exposure of each pixel also allows for an extremely wide dynamic range that can encompass typical natural and artificially illuminated scenes, with sample night-time scans achieving full-spectrum peak-to-peak dynamic ranges of > 50,000:1.

Conclusions: This system's adaptability, cost-effectiveness and open-source nature position it as a valuable tool for researchers investigating the complex relationships between light, environment, behaviour, ecology and biodiversity, with further potential uses in many other fields.

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高光谱开源成像仪(HOSI)。
背景:光环境的空间和光谱特性支撑着动物行为、生态和进化的许多方面,对这些信息进行量化在光学物理学、农业/植物科学、人类心理物理学、食品科学、建筑和材料科学等领域至关重要。夜间人造光(ALAN)的威胁不断升级,为测量光污染的视觉影响提出了独特的挑战,需要在人类可见光和紫外线范围内的低光水平下,在所有视角下进行测量,并且通常具有高场景内对比度。在这里,我提出了一个高光谱开源成像仪(HOSI),一个创新和低成本的解决方案,用于收集全场高光谱数据。系统使用Hamamatsu C12880MA微型光谱仪进行单点测量,连同用于空间控制的机动框架。硬件使用现成的部件和3D打印部件,总成本约为350英镑。该系统可以直接从计算机或智能手机上运行,具有图形用户界面,使其高度便携和用户友好。HOSI系统可以拍摄满足ALAN研究困难要求的全景高光谱图像,对0.001 cd.m-2左右的弱光敏感,在320-880 nm范围内,光谱分辨率为~ 9 nm (FWHM),空间分辨率为~ 2周期/度。每个像素的独立曝光也允许一个非常宽的动态范围,可以包含典型的自然和人工照明场景,样本夜间扫描实现全光谱峰对峰动态范围bbb50,000:1。结论:该系统的适应性、成本效益和开源特性使其成为研究人员研究光、环境、行为、生态和生物多样性之间复杂关系的宝贵工具,并在许多其他领域具有进一步的潜在用途。
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来源期刊
BMC Biology
BMC Biology 生物-生物学
CiteScore
7.80
自引率
1.90%
发文量
260
审稿时长
3 months
期刊介绍: BMC Biology is a broad scope journal covering all areas of biology. Our content includes research articles, new methods and tools. BMC Biology also publishes reviews, Q&A, and commentaries.
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