Galangin synergistically revives the antibacterial activity of vancomycin against vancomycin-resistant Enterococcus faecium.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of Applied Microbiology Pub Date : 2025-01-06 DOI:10.1093/jambio/lxaf011

Intu-Orn Ayamuang, Yothin Teethaisong, Kittipot Sirichaiwetchakoon, Siriporn Suknasang, Santi Watthana, Yupaporn Chaiseha, Griangsak Eumkeb

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Abstract

Aims: Enterococcus faecium is one of the most important opportunistic pathogens threatening human health worldwide. Resistance to vancomycin (VAN) is increasing at an alarming rate. Resurrecting antibiotics using a combination approach is a promising alternative avenue. Galangin (GAL) is a bioactive compound constituted in herbal plants. This study aimed to evaluate the synergistic activity of the combination of GAL and VAN and mode of action against vancomycin-resistant E. faecium (VREfm) strains.

Methods and results: The minimal inhibitory concentrations against these bacteria were 8-64 μg ml-1 for VAN and 512 μg ml-1 for GAL. The VAN plus GAL combination exhibited synergistic effects against E. faecium isolates, with a fraction inhibitory concentration index of 0.26-0.28. Time-kill assays confirmed this synergism. Mechanistic studies showed that the combination induced intracellular constituent leakage, suggesting impaired membrane permeability and electron microscopy revealed peptidoglycan and membrane damage. Additionally, the GAL plus VAN combination inhibited biofilm formation and significantly reduced lipid, protein, and carbohydrate contents, as shown by Fourier-transform infrared spectroscopy (FTIR).

Conclusions: GAL could reverse the activity of VAN against VREfm by damaging bacterial cell envelope, inhibiting biofilm formation, and reducing biomolecule contents, emphasizing its potential as a valuable adjunct to VAN in treating VREfm infections.

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高良姜协同恢复万古霉素对耐万古霉素屎肠球菌的抗菌活性。

目的：粪肠球菌是威胁人类健康的重要条件致病菌之一。对万古霉素（VAN）的耐药性正以惊人的速度增长。使用联合方法复活抗生素是一种有希望的替代途径。高良姜素（Galangin， GAL）是一种存在于草本植物中的生物活性化合物。本研究旨在评价GAL与VAN联合用药对万古霉素耐药粪肠杆菌（VREfm）的协同作用及其作用方式。方法与结果：VAN和GAL的最低抑菌浓度分别为8 ~ 64 μg ml-1和512 μg ml-1， VAN + GAL对粪肠杆菌具有协同抑菌作用，抑菌浓度指数为0.26 ~ 0.28。时间杀伤试验证实了这种协同作用。机制研究表明，该组合引起细胞内成分渗漏，提示膜通透性受损，电镜显示肽聚糖和膜损伤。此外，傅里叶变换红外光谱（FTIR）显示，GAL + VAN组合抑制了生物膜的形成，显著降低了脂质、蛋白质和碳水化合物的含量。结论：GAL可以通过破坏细菌细胞包膜、抑制生物膜形成和降低生物分子含量来逆转VAN对VREfm的活性，强调其作为VAN治疗VREfm感染的有价值的辅助药物的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.