Integrated UHPLC-mS/mS analysis, spectrum-effect relationship, and molecular docking to study the antidiabetic components of Cyclocarya paliurus.

IF 1.6 3区化学 Q3 CHEMISTRY, APPLIED Natural Product Research Pub Date : 2025-01-10 DOI:10.1080/14786419.2024.2448200

Dan-Dan Zhang, Ming-Hua Hu, Zhen Luo, Yu-Ying Yang, Hui-Jun Li, Xin-Yao Luo, Qiong Wei, Xiao-Chuan Ye

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Abstract

The leaves of Cyclocarya paliurus (Batal) Iljinsk., a plant native to China that has long been used in traditional Chinese medicine to treat diabetes. It remains to be determined what chemical constituents are responsible for this effect. The aim of this study was to identify the antidiabetic components of C. paliurus using the spectrum-effect relationship and confirmed using molecular docking. The components of C. paliurus were detected using ultra-high-performance liquid chromatography mass spectrometry/mass spectrometry (UHPLC-MS/MS) and ultra-high-performance liquid chromatography-diode array detector (UHPLC-DAD). C. paliurus could significantly reduce glycosylated haemoglobin, blood glucose levels and blood lipid profile. Esculetin, shikimic acid, isoquercitrin, quercitrin, kaempferol-3-O-rhamnoside, tricin, and pterocaryoside A were determined to be the main antidiabetic components of C. paliurus. Results revealed that the antidiabetic effect of C. paliurus was due to the combination of multiple chemical components. The method developed is valuable for identifying the active ingredients of this plant species.

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综合UHPLC-mS/mS分析、光谱效应关系、分子对接等方法研究青环草抗糖尿病成分。

伊尔金斯克环叶青（Batal）的叶子。这是一种原产于中国的植物，长期以来一直被用于治疗糖尿病。究竟是什么化学成分造成了这种效果还有待确定。本研究的目的是利用光谱效应关系对黄菖蒲的抗糖尿病成分进行鉴定，并进行分子对接验证。采用超高效液相色谱-质谱联用技术（UHPLC-MS/MS）和超高效液相色谱-二极管阵列检测器（UHPLC-DAD）对黄菖蒲的成分进行检测。黄菖蒲能显著降低糖化血红蛋白、血糖水平和血脂水平。槲皮素、石草酸、异槲皮素、槲皮素、山奈酚-3- o -鼠李糖苷、tricin和翼石桃苷A是苦姜的主要抗糖尿病成分。结果表明，苍尾草的抗糖尿病作用是多种化学成分共同作用的结果。该方法对鉴别该植物的有效成分具有一定的参考价值。

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.