Genetic diversity and population structure analysis of short-day onions using molecular markers in association with resistance to Fusarium basal rot.

IF 5.4 2区生物学 Q1 PLANT SCIENCES Physiologia plantarum Pub Date : 2025-01-01 DOI:10.1111/ppl.70042

SaeidReza Poursakhi, Hossein Ali Asadi-Gharneh, Mehdi Nasr-Esfahani, Zahra Abbasi, Hamed Hassanzadeh Khankahdani

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Abstract

In this research, we analyzed Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeats (ISSR) and Sequence-related amplified polymorphism (SRAP) markers to evaluate the genetic diversity of eighteen different onion genotypes with various resistant levels to FOC. The results showed that the polymorphism means between RAPD primers was 61.11 to 81.81%; ISSR primers, 62.50 to 81.81%; and SRAP primers, 56.25 to 76.25%. Overall, by assessing MI, PIC, I and H indices, indicating the best thrive in evaluating the genetic diversity of the related onion populations. There is a significant correlation between the generated dendrograms based on similarity matrices. The classification pattern in dendrograms shows a corresponding correlation with the FOC disease severity bunches. So in all three markers studied, 'Saba' and 'Saba - HS', the most resistant ones to FOC disease, were grouped in a branch, and the 'Sahar - HS' and 'Golden Eye', the most susceptible ones were also grouped in another branch separately. This finding indicates that predominant primers act as markers linked to resistance gene(s) against FOC, which can be used to select onions resistant to FOC disease in any breeding scheme.

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短日洋葱抗枯萎病遗传多样性及群体结构分析。

本研究利用随机扩增多态性DNA （RAPD）、简单重复序列（ISSR）和序列相关扩增多态性（SRAP）标记，对18个不同洋葱抗FOC水平基因型的遗传多样性进行了评价。结果表明，RAPD引物间多态性均值为61.11% ~ 81.81%；ISSR引物占62.50% ~ 81.81%；SRAP引物为56.25 ~ 76.25%。总体而言，通过对MI、PIC、I和H指数的评价，表明对相关洋葱群体的遗传多样性评价效果最好。基于相似矩阵生成的树形图之间存在显著的相关性。树状图的分类模式显示出与FOC疾病严重程度束的相应相关性。因此，在所研究的所有三个标记中，对FOC病抵抗力最强的“Saba”和“Saba - HS”被分组在一个分支中，而最易感的“Sahar - HS”和“Golden Eye”也分别分组在另一个分支中。这一发现表明，优势引物可作为FOC抗性基因的连锁标记，可用于在任何育种方案中选择对FOC病具有抗性的洋葱。

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来源期刊

Physiologia plantarum 生物-植物科学

CiteScore

11.00

自引率

3.10%

发文量

224

审稿时长

3.9 months

期刊介绍： Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.