Discovery of Novel Thiazolohydrazone Derivatives as an Alternative Option in the Treatment of Zoonotic Toxocara canis: In Vitro and In Silico Evaluation.

Abstract

Thiazoles serve as pharmacophores in many molecules that exhibit significant biological activity. This study used in vitro assays and in silico methodologies to identify novel medication candidates for preclinical evaluation of visceral toxocariasis treatment. The objective was to assess the impact of 11 thiazolohydrazone derivative compounds on the larval eggs of Toxocara canis. In addition, the inhibitory effects of these compounds on IL-6 levels in THP-1 cells stimulated with larval eggs have been examined. Compounds indicating the most significant inhibitory effect on IL-6 and a suppressive effect on larval eggs have been included in molecular dynamics studies. The study revealed that, when evaluating all thiazolohydrazone derivatives together, Compounds 3g and 3h possessed the highest antiparasitic efficiency, encouraging further molecular dynamics investigations on these compounds. Consequently, it can be theorised that if the results from this study are corroborated by in vivo studies, new anti-nematodal medication candidates may be identified.