PlomBOX: a low cost bioassay for the sensitive detection of lead in drinking water.

Communications engineering Pub Date : 2025-01-07 DOI:10.1038/s44172-024-00337-7

A Dias, M Alvarez, Y Gándola, A Deisting, E Alba Posse, H Arnaldi, H Asorey, X Bertou, A Colque, F Favela-Pérez, J Gasulla, M Gómez Berisso, J O Guerra-Pulido, J Lipovetzky, J Lobera, M B Lovino, L Marpegan, D Martín, S Mejía Muñoz, J Monroe, A D Nadra, R Pregliasco, G Rumi, A Rossen, M Tallis, A Thompson, M Triana, M L Vazquez Miranda

{"title":"PlomBOX: a low cost bioassay for the sensitive detection of lead in drinking water.","authors":"A Dias, M Alvarez, Y Gándola, A Deisting, E Alba Posse, H Arnaldi, H Asorey, X Bertou, A Colque, F Favela-Pérez, J Gasulla, M Gómez Berisso, J O Guerra-Pulido, J Lipovetzky, J Lobera, M B Lovino, L Marpegan, D Martín, S Mejía Muñoz, J Monroe, A D Nadra, R Pregliasco, G Rumi, A Rossen, M Tallis, A Thompson, M Triana, M L Vazquez Miranda","doi":"10.1038/s44172-024-00337-7","DOIUrl":null,"url":null,"abstract":"<p><p>This paper reports the design of a biosensor for sensitive, low-cost measurement of lead in drinking water. The biosensor uses a genetically-modified strain of Escherichia coli, which serves as both signal amplifier and reporter of lead in water, measured via colour change. We developed the PlomBOX measurement platform to image this colour change and we demonstrate its capability to detect concentrations as low as the World Health Organisation upper limit for drinking water of 10 ppb. Our approach does not require expensive infrastructure or expert operators, and its automated sensing, detection and result visualisation platform is user-friendly and robust compared to existing lead biosensors-critical features to enable measurement by non-experts at the point of use.</p>","PeriodicalId":72644,"journal":{"name":"Communications engineering","volume":"4 1","pages":"2"},"PeriodicalIF":0.0000,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11707191/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Communications engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1038/s44172-024-00337-7","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

This paper reports the design of a biosensor for sensitive, low-cost measurement of lead in drinking water. The biosensor uses a genetically-modified strain of Escherichia coli, which serves as both signal amplifier and reporter of lead in water, measured via colour change. We developed the PlomBOX measurement platform to image this colour change and we demonstrate its capability to detect concentrations as low as the World Health Organisation upper limit for drinking water of 10 ppb. Our approach does not require expensive infrastructure or expert operators, and its automated sensing, detection and result visualisation platform is user-friendly and robust compared to existing lead biosensors-critical features to enable measurement by non-experts at the point of use.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

PlomBOX：一种低成本的生物测定法，用于敏感检测饮用水中的铅。

本文报道了一种用于饮用水中铅的灵敏、低成本测量的生物传感器的设计。这种生物传感器使用了一种转基因大肠杆菌菌株，它既是信号放大器，又是水中铅的报告者，通过颜色变化来测量。我们开发了PlomBOX测量平台来对这种颜色变化进行成像，并展示了其检测浓度低至世界卫生组织饮用水上限10 ppb的能力。我们的方法不需要昂贵的基础设施或专家操作人员，与现有的铅生物传感器相比，其自动传感、检测和结果可视化平台对用户友好且强大，这些关键功能使非专家能够在使用点进行测量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Communications engineering

自引率

0.00%

发文量