A novel electrochemical biosensor based on TiO₂ nanotube array films for highly sensitive detection of exosomes.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL Talanta Pub Date : 2025-01-06 DOI:10.1016/j.talanta.2025.127545

Liqi He, Guanghai Piao, Xu Yin, Jie Feng, Ting Zhang, Caiwei Hu, Yu Bai, Ji Man Kim, Mingshi Jin

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引用次数: 0

Abstract

Exosomes have emerged as a powerful biomarker for early cancer diagnosis, however, accurately detecting cancer-derived exosomes in biofluids remains a crucial challenge. In this study, we present a novel label-free electrochemical biosensor utilizing titanium dioxide nanotube array films (TiO₂NTAs) for the sensitive detection of exosomes in complex biological samples. This innovative biosensor takes advantage of the excellent electrochemical properties of TiO₂NTAs and their specific interactions with the phosphate groups of exosomes. The transport of ions and electrons within the exosome-captured TiO₂ nanotubes is hindered, leading to a significant alteration in the electrochemical response signal and enabling highly sensitive detection of exosomes. Consequently, the biosensor demonstrates a wide linear detection range from 5 × 10¹ to 1 × 10⁷ particles/μL with a limit of detection of 12.7 particles/μL and 12.6 particles/μL for the exosomes derived from hepatocellular carcinoma and colon cancer cells, respectively. Furthermore, the TiO₂NTAs biosensor can successfully distinguish the signal of extracellular vesicles in real human serum samples between 20 hepatocellular carcinoma, 20 colon cancer and 20 healthy persons (p < 0.0001). This method had a promising potential in biochemical analysis and clinical cancer diagnosis.

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.