Applicability of intI1 as an indicator gene for securing the removal efficiency of extracellular antimicrobial resistance genes in full-scale wastewater treatment plants.

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Bioresource Technology Pub Date : 2025-01-08 DOI:10.1016/j.biortech.2025.132047

Rongxuan Wang, Mardalisa, Hiroe Hara-Yamamura, Norihisa Matsuura, Ryo Honda

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Abstract

Mitigating the release of extracellular antimicrobial resistance genes (exARGs) from wastewater treatment plants (WWTPs) is crucial for preventing the spread of antimicrobial resistance from human domains into the environment. This study aimed to evaluate the applicability of intI1 as a performance indicator for securing the removal of exARGs at WWTPs. We investigated the reduction of exARGs and intI1 in a full-scale WWTP, where identical wastewater was treated using conventional activated sludge (CAS) and membrane bioreactor (MBR) systems. The log reduction values (LRVs) for exARGs were lower than those for intracellular ARGs (iARGs) across all ARG species and treatment systems. LRVs for exARGs were consistently higher in the MBR than in the CAS. The intI1 exhibited lower LRVs compared to most exARGs, ensuring a minimum LRV of exARG in both CAS and MBR systems. Consequently, intI1 is an effective indicator gene for securing the removal of exARGs.

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intI1作为指示基因在污水处理厂中确保细胞外抗菌素耐药基因去除效率的适用性

减少废水处理厂（WWTPs）的细胞外抗菌素耐药基因（exARGs）的释放对于防止抗菌素耐药性从人类领域传播到环境中至关重要。本研究旨在评估intI1作为确保污水处理厂去除exARGs的性能指标的适用性。我们研究了一个大规模污水处理厂中exARGs和intI1的减少情况，在该污水处理厂中，使用传统活性污泥（CAS）和膜生物反应器（MBR）系统处理相同的废水。在所有ARG种类和处理系统中，exARGs的对数还原值（lrv）低于细胞内ARGs （iARGs）。exARGs的lrv在MBR中始终高于CAS。与大多数exARG相比，intI1表现出较低的LRV，确保了CAS和MBR系统中exARG的最小LRV。因此，intI1是确保exARGs去除的有效指示基因。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.