{"title":"Immobilization of 4-MBA & Cu<sup>2+</sup> on Au nanoparticles modified screen-printed electrode for glyphosate detection.","authors":"Wei Li, Hongqi Wu, Cong Wu, Peidong Jiao, Ling Xu, Haiyan Song","doi":"10.1016/j.talanta.2025.127530","DOIUrl":null,"url":null,"abstract":"<p><p>This study introduces an innovative electrochemical biosensor, engineered through the functionalization screen-printed electrode (SPE) with a coordination complex comprised of 4-mercaptobenzoic acid (4-MBA) and copper ions (Cu<sup>2+</sup>), achieving precise quantitative determination of glyphosate. Electrodepositing gold nanoparticles (AuNPs) onto the electrode surface, forming a self-assembled monolayer (SAM) of 4-MBA via thiol-gold interactions, and immobilizing Cu<sup>2+</sup> via coordination bonding with the monolayer, finalizing the electrochemical biosensor construction as Cu<sup>2+</sup>/4-MBA/AuNPs/SPE. The successful modification of the biosensor interface is confirmed through scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and electrochemical characterization. Through parameter optimization, critical metrics for the biosensor preparation process have been determined. Using square wave voltammetry (SWV), a linear relationship between the glyphosate concentration and the peak current inhibition ratio at the electrode surface is established. Additionally, the repeatability and anti-interference capabilities of the fabricated biosensors are evaluated. The experimental outcomes affirm the biosensor's capability for quantitative glyphosate detection across a 5-100 nM range, boasting a 1.65 nM limit of detection (LOD). Testing on tap water samples verifies a robust recovery rate for glyphosate residues, spanning 89.84 %-107.48 %. The proposed biosensor holds significant promise for glyphosate detection, offering substantial applicability and this study provides a valuable reference for the advancement of biosensors geared toward the quantitative assessment of organophosphate pesticides (OPs).</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127530"},"PeriodicalIF":5.6000,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Talanta","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.talanta.2025.127530","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
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Abstract
This study introduces an innovative electrochemical biosensor, engineered through the functionalization screen-printed electrode (SPE) with a coordination complex comprised of 4-mercaptobenzoic acid (4-MBA) and copper ions (Cu2+), achieving precise quantitative determination of glyphosate. Electrodepositing gold nanoparticles (AuNPs) onto the electrode surface, forming a self-assembled monolayer (SAM) of 4-MBA via thiol-gold interactions, and immobilizing Cu2+ via coordination bonding with the monolayer, finalizing the electrochemical biosensor construction as Cu2+/4-MBA/AuNPs/SPE. The successful modification of the biosensor interface is confirmed through scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and electrochemical characterization. Through parameter optimization, critical metrics for the biosensor preparation process have been determined. Using square wave voltammetry (SWV), a linear relationship between the glyphosate concentration and the peak current inhibition ratio at the electrode surface is established. Additionally, the repeatability and anti-interference capabilities of the fabricated biosensors are evaluated. The experimental outcomes affirm the biosensor's capability for quantitative glyphosate detection across a 5-100 nM range, boasting a 1.65 nM limit of detection (LOD). Testing on tap water samples verifies a robust recovery rate for glyphosate residues, spanning 89.84 %-107.48 %. The proposed biosensor holds significant promise for glyphosate detection, offering substantial applicability and this study provides a valuable reference for the advancement of biosensors geared toward the quantitative assessment of organophosphate pesticides (OPs).
期刊介绍：
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
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