Aptamer proximal enzyme cascade reactions for ultrafast detection of glucose in human blood serum

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-01-13 DOI:10.1007/s00604-024-06935-8

Min Liu, Chuanlin Zhu, Zihe Dong, Zhangmin Wang, Huan Yang, Jie Li, Ke Li, Bo Shen, Xinmin Li, Ping Leng, Shijia Ding, Jinlin Guo, Juan Zhang

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Abstract

An innovative colorimetric sensing strategy was developed for the detection of glucose by the integration of glucose aptamer, glucose oxidase (GOx), and horseradish peroxidase (HRP), termed aptamer proximal enzyme cascade reactions (APECR). In the presence of glucose, aptamer binding enables GOx to catalyze glucose oxidation into H₂O₂ efficiently. Subsequently, the adjacent HRP catalyzes the oxidation of the peroxidase substrate, 2,2′-biazobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS²⁻), utilizing the generated H₂O₂, resulting in a distinct color change. In comparison to the free enzymes and the HRP-GOx system, APECR exhibited higher colorimetric signal. This approach achieved glucose detection within three minutes, which was significantly faster than previous methods. This method showed good sensitivity and selectivity with a limit of detection of 0.013 mM. Moreover, the practical utility of this strategy was verified by achieving rapid detection of glucose in clinical serum samples. Hence, the developed strategy has the advantages of simple operation and rapid analysis time for the detection of glucose in human serum.

Graphical Abstract

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适体近端酶级联反应超快速检测人血清中葡萄糖。

通过整合葡萄糖适体、葡萄糖氧化酶（GOx）和辣根过氧化物酶（HRP），开发了一种创新的比色传感策略，用于检测葡萄糖，称为适体近端酶级联反应（APECR）。在葡萄糖存在的情况下，适体结合使GOx能够有效地催化葡萄糖氧化成H2O2。随后，相邻的HRP利用生成的H2O2催化过氧化物酶底物2,2′-偶氮唑（3-乙基苯并噻唑-6-磺酸）（ABTS2-）氧化，产生明显的颜色变化。与游离酶和HRP-GOx体系相比，APECR表现出更高的比色信号。该方法在3分钟内实现了葡萄糖检测，比以前的方法明显快。该方法具有良好的灵敏度和选择性，检出限为0.013 mM。此外，通过实现临床血清样品中葡萄糖的快速检测，验证了该策略的实用性。因此，该方法具有操作简单、分析时间短等优点，可用于人血清中葡萄糖的检测。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.