{"title":"Pink-colored Cypridina luciferase from Cypridina (Vargula) hilgendorfii: Oxygen-triggered luminescence reaction following NAD(P)H-FMN flavin reductase reaction","authors":"Satoshi Inouye","doi":"10.1016/j.bbrc.2024.151285","DOIUrl":null,"url":null,"abstract":"<div><div>The pink-colored Cypridina luciferase (CypLase∗) from <em>Cypridina</em> (<em>Vargula</em>) <em>hilgendorfii</em> contains an unknown chromophore (CypL∗), derived from Cypridina luciferin (CypL). When CypLase∗ was treated with NAD(P)H-FMN flavin reductase (FRase) and NADH, the luminescence intensity in the reaction mixture increased significantly after gentle tapping. This observation suggests that CypL∗ in CypLase is enzymatically converted to CypL by the reduced flavin (FMNH<sub>2</sub>) through the FRase reaction, and the resulting complex of CypL and CypLase reacts with O<sub>2</sub> to emit light. Therefore, CypL∗ might be a dehydrogenated form of CypL and might serve as another storage form in <em>Cypridina</em> specimens.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"747 ","pages":"Article 151285"},"PeriodicalIF":2.5000,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical and biophysical research communications","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0006291X24018217","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The pink-colored Cypridina luciferase (CypLase∗) from Cypridina (Vargula) hilgendorfii contains an unknown chromophore (CypL∗), derived from Cypridina luciferin (CypL). When CypLase∗ was treated with NAD(P)H-FMN flavin reductase (FRase) and NADH, the luminescence intensity in the reaction mixture increased significantly after gentle tapping. This observation suggests that CypL∗ in CypLase is enzymatically converted to CypL by the reduced flavin (FMNH2) through the FRase reaction, and the resulting complex of CypL and CypLase reacts with O2 to emit light. Therefore, CypL∗ might be a dehydrogenated form of CypL and might serve as another storage form in Cypridina specimens.
期刊介绍：
Biochemical and Biophysical Research Communications is the premier international journal devoted to the very rapid dissemination of timely and significant experimental results in diverse fields of biological research. The development of the "Breakthroughs and Views" section brings the minireview format to the journal, and issues often contain collections of special interest manuscripts. BBRC is published weekly (52 issues/year).Research Areas now include: Biochemistry; biophysics; cell biology; developmental biology; immunology
; molecular biology; neurobiology; plant biology and proteomics
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