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Corrigendum to "Plasmodium falciparum Raf kinase inhibitor is a lipid binding protein that interacts with and regulates the activity of PfCDPK1, an essential plant-like kinase required for red blood cell invasion" [Biochem. Biophys. Res. Commun. 749 (2025) 151350]. 恶性疟原虫Raf激酶抑制剂是一种脂质结合蛋白,与PfCDPK1相互作用并调节PfCDPK1的活性,PfCDPK1是红细胞入侵所需的一种必需的植物样激酶[Biochem]。Biophys。法典第749(2025)151350条]。
IF 2.2 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-26 Epub Date: 2026-02-02 DOI: 10.1016/j.bbrc.2026.153375
Manish Sharma, Deepak Krishnan, Ayushi Singh, Pooja Negi, Komal Rani, Amjesh Revikumar, Manoj Munde, Abhisheka Bansal
{"title":"Corrigendum to \"Plasmodium falciparum Raf kinase inhibitor is a lipid binding protein that interacts with and regulates the activity of PfCDPK1, an essential plant-like kinase required for red blood cell invasion\" [Biochem. Biophys. Res. Commun. 749 (2025) 151350].","authors":"Manish Sharma, Deepak Krishnan, Ayushi Singh, Pooja Negi, Komal Rani, Amjesh Revikumar, Manoj Munde, Abhisheka Bansal","doi":"10.1016/j.bbrc.2026.153375","DOIUrl":"10.1016/j.bbrc.2026.153375","url":null,"abstract":"","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":" ","pages":"153375"},"PeriodicalIF":2.2,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146112171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corrigendum to "Suppressing Cyclooxygenase-2 Prevents nonalcoholic and inhibits apoptosis of hepatocytes that are involved in the Akt/p53 signal pathway" [Biochem. Biophys. Res. Commun. 469 (2016) 1034-1040]. “抑制环氧化酶-2防止非酒精性和抑制参与Akt/p53信号通路的肝细胞凋亡”[Biochem]的更正。Biophys。共同判例,469 (2016)1034-1040 [j]。
IF 2.2 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-26 Epub Date: 2026-01-27 DOI: 10.1016/j.bbrc.2026.153299
Jialing Wu, Chong Chen, Xi Hu, Xianbin Cai, Yinghong Guan, Hui Hu, Qinjia Wang, Xiaofeng Chen, Bozhi Cai, Xubin Jing
{"title":"Corrigendum to \"Suppressing Cyclooxygenase-2 Prevents nonalcoholic and inhibits apoptosis of hepatocytes that are involved in the Akt/p53 signal pathway\" [Biochem. Biophys. Res. Commun. 469 (2016) 1034-1040].","authors":"Jialing Wu, Chong Chen, Xi Hu, Xianbin Cai, Yinghong Guan, Hui Hu, Qinjia Wang, Xiaofeng Chen, Bozhi Cai, Xubin Jing","doi":"10.1016/j.bbrc.2026.153299","DOIUrl":"https://doi.org/10.1016/j.bbrc.2026.153299","url":null,"abstract":"","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"802 ","pages":"153299"},"PeriodicalIF":2.2,"publicationDate":"2026-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146131133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SLL-023ACP, a KOR/MOR dual agonist, produces potent antinociception with fewer side effects SLL-023ACP是一种KOR/MOR双激动剂,产生有效的抗孕毒作用,副作用少
IF 2.2 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-05 DOI: 10.1016/j.bbrc.2026.153411
Yi-Quan Shen , Pan-Wen Liu , Deng-Gao Zhang , Min Liu , Jia-Wen Luo , Yu-Liang Lin , Jiang-Wen Gui , Li-Jin Feng , Jing-Gen Liu , Wei Li , Yu-Jun Wang
Opioids have served as the first-line treatment for pain management. However, their side effects such as addiction potential and constipation have limited their clinical use. Here, we characterized a novel 4,5-epoxymorphinan-based derivative SLL-023ACP, which we previously identified as a high affinity ligand towards κ-opioid receptor (KOR) and μ-opioid receptor (MOR). SLL-023ACP was identified as a full KOR agonist (EC50 = 12.84 nM, Emax = 85.69%) and MOR partial agonist (EC50 = 31.21 nM, Emax = 54.49%) in vitro functional cAMP inhibition assay. In vivo, SLL-023ACP displayed potent antinociceptive effects with ED50 values of 2.6 mg/kg in the hot plate test and 0.15 mg/kg in the abdominal constriction test, exhibiting greater potency than KOR agonist U50,488H and MOR agonist Morphine. Its antinociceptive action is effectively reversed by the non-selective opioid antagonist naloxone and the MOR antagonist CTAP in the hot plate test, and by the KOR antagonist nor-BNI in the abdominal constriction test, indicating its analgesic effects was via MOR and KOR. Importantly, at analgesic doses (2.6 and 5 mg/kg), SLL-023ACP induced no conditioned place preference (CPP) and caused less constipation than morphine, although it did produce significant sedation. Taken together, these results suggest that SLL-023ACP is a potent dual KOR/MOR agonist with a favorable profile of potent analgesia with reduced addiction liability and gastrointestinal dysfunction.
{"title":"SLL-023ACP, a KOR/MOR dual agonist, produces potent antinociception with fewer side effects","authors":"Yi-Quan Shen ,&nbsp;Pan-Wen Liu ,&nbsp;Deng-Gao Zhang ,&nbsp;Min Liu ,&nbsp;Jia-Wen Luo ,&nbsp;Yu-Liang Lin ,&nbsp;Jiang-Wen Gui ,&nbsp;Li-Jin Feng ,&nbsp;Jing-Gen Liu ,&nbsp;Wei Li ,&nbsp;Yu-Jun Wang","doi":"10.1016/j.bbrc.2026.153411","DOIUrl":"10.1016/j.bbrc.2026.153411","url":null,"abstract":"<div><div>Opioids have served as the first-line treatment for pain management. However, their side effects such as addiction potential and constipation have limited their clinical use. Here, we characterized a novel 4,5-epoxymorphinan-based derivative SLL-023ACP, which we previously identified as a high affinity ligand towards κ-opioid receptor (KOR) and μ-opioid receptor (MOR). SLL-023ACP was identified as a full KOR agonist (EC<sub>50</sub> = 12.84 nM, E<sub>max</sub> = 85.69%) and MOR partial agonist (EC<sub>50</sub> = 31.21 nM, E<sub>max</sub> = 54.49%) <em>in vitro</em> functional cAMP inhibition assay. <em>In vivo</em>, SLL-023ACP displayed potent antinociceptive effects with ED<sub>50</sub> values of 2.6 mg/kg in the hot plate test and 0.15 mg/kg in the abdominal constriction test, exhibiting greater potency than KOR agonist U50,488H and MOR agonist Morphine. Its antinociceptive action is effectively reversed by the non-selective opioid antagonist naloxone and the MOR antagonist CTAP in the hot plate test, and by the KOR antagonist nor-BNI in the abdominal constriction test, indicating its analgesic effects was via MOR and KOR. Importantly, at analgesic doses (2.6 and 5 mg/kg), SLL-023ACP induced no conditioned place preference (CPP) and caused less constipation than morphine, although it did produce significant sedation. Taken together, these results suggest that SLL-023ACP is a potent dual KOR/MOR agonist with a favorable profile of potent analgesia with reduced addiction liability and gastrointestinal dysfunction.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"806 ","pages":"Article 153411"},"PeriodicalIF":2.2,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146122735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Engineering chain-branched bispecific VHH complexes ("Dendrobodies") using SpyCatcher and SnoopCatcher pairings. 利用SpyCatcher和SnoopCatcher配对构建链支双特异性VHH复合物(“树突体”)。
IF 2.2 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-05 DOI: 10.1016/j.bbrc.2026.153413
Kota Kurogane, Miyuu Sekine, Ryutaro Asano, Shigekazu Yano, Koki Makabe

Bispecific antibodies (bsAbs) that direct immune cells toward tumor cells have substantial therapeutic potential. However, bsAbs produced by tandemly linking single-domain antibodies (VHHs) often exhibit reduced activity because the N-terminal region of each VHH, which contains the complementarity-determining regions (CDR) loops, becomes sterically hindered by the adjacent domain. To address this issue, we developed a branched VHH complex architecture, termed Dendrobody, in which VHHs are joined through their C-terminal sides using orthogonal SpyCatcher/SpyTag and SnoopCatcher/SnoopTag systems. This design enables site-specific covalent assembly of multiple VHHs while preserving accessibility of their antigen-binding regions. We generated a bispecific anti-CD3/anti-EGFR Dendrobody (Dendrobody-CD3/EGFR) through a one-pot ligation of three recombinant components. The purified complex displayed correct folding with a thermal transition temperature (Tm) of 56 °C. Biolayer interferometry showed strong EGFR binding, and the Dendrobody triggered selective T-cell-mediated cytotoxicity toward EGFR-positive cancer cells. No cytotoxicity was detected in EGFR-negative cells, confirming antigen specificity. In addition, the platform allowed straightforward production of another variant, Dendrobody-CD16/EGFR, demonstrating its modularity. The Dendrobody framework thus offers an alternative strategy for constructing bispecific and multispecific VHH-based therapeutics with minimal steric interference and high functional adaptability.

{"title":"Engineering chain-branched bispecific VHH complexes (\"Dendrobodies\") using SpyCatcher and SnoopCatcher pairings.","authors":"Kota Kurogane, Miyuu Sekine, Ryutaro Asano, Shigekazu Yano, Koki Makabe","doi":"10.1016/j.bbrc.2026.153413","DOIUrl":"https://doi.org/10.1016/j.bbrc.2026.153413","url":null,"abstract":"<p><p>Bispecific antibodies (bsAbs) that direct immune cells toward tumor cells have substantial therapeutic potential. However, bsAbs produced by tandemly linking single-domain antibodies (VHHs) often exhibit reduced activity because the N-terminal region of each VHH, which contains the complementarity-determining regions (CDR) loops, becomes sterically hindered by the adjacent domain. To address this issue, we developed a branched VHH complex architecture, termed Dendrobody, in which VHHs are joined through their C-terminal sides using orthogonal SpyCatcher/SpyTag and SnoopCatcher/SnoopTag systems. This design enables site-specific covalent assembly of multiple VHHs while preserving accessibility of their antigen-binding regions. We generated a bispecific anti-CD3/anti-EGFR Dendrobody (Dendrobody-CD3/EGFR) through a one-pot ligation of three recombinant components. The purified complex displayed correct folding with a thermal transition temperature (Tm) of 56 °C. Biolayer interferometry showed strong EGFR binding, and the Dendrobody triggered selective T-cell-mediated cytotoxicity toward EGFR-positive cancer cells. No cytotoxicity was detected in EGFR-negative cells, confirming antigen specificity. In addition, the platform allowed straightforward production of another variant, Dendrobody-CD16/EGFR, demonstrating its modularity. The Dendrobody framework thus offers an alternative strategy for constructing bispecific and multispecific VHH-based therapeutics with minimal steric interference and high functional adaptability.</p>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"806 ","pages":"153413"},"PeriodicalIF":2.2,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146140835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ERA1 is essential for meristem growth in Arabidopsis thaliana ERA1对拟南芥分生组织生长至关重要
IF 2.2 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-05 DOI: 10.1016/j.bbrc.2026.153412
Jingwen He , Yuang Ma , Yao Jiang, Zengxiu Feng, Shiquan Yang
Meristematic tissue is essential for plant growth and development, serving as a fundamental component in these processes. However, the regulatory networks and mechanism involved in its function remain inadequately understood. In this study, we investigated the role of the farnesyltransferase ERA1 in the development of plant meristematic tissues through phenotypic analysis, in situ hybridization and cell biology techniques. Our findings revealed that era1-11 mutant exhibited retarded growth and impaired development of meristematic tissues. Moreover, the expression levels of key genes associated with meristematic tissue development, such as WUS, CLV3, PLT, were significantly altered in this mutant. In summary, ERA1 plays a vital role in the development of plant meristematic tissues by participating in multiple signaling pathways, thus contributing new insights into the regulatory network involved in plant meristematic tissue development.
{"title":"ERA1 is essential for meristem growth in Arabidopsis thaliana","authors":"Jingwen He ,&nbsp;Yuang Ma ,&nbsp;Yao Jiang,&nbsp;Zengxiu Feng,&nbsp;Shiquan Yang","doi":"10.1016/j.bbrc.2026.153412","DOIUrl":"10.1016/j.bbrc.2026.153412","url":null,"abstract":"<div><div>Meristematic tissue is essential for plant growth and development, serving as a fundamental component in these processes. However, the regulatory networks and mechanism involved in its function remain inadequately understood. In this study, we investigated the role of the farnesyltransferase ERA1 in the development of plant meristematic tissues through phenotypic analysis, in situ hybridization and cell biology techniques. Our findings revealed that <em>era1-11</em> mutant exhibited retarded growth and impaired development of meristematic tissues. Moreover, the expression levels of key genes associated with meristematic tissue development, such as <em>WUS</em>, <em>CLV3</em>, <em>PLT</em>, were significantly altered in this mutant. In summary, ERA1 plays a vital role in the development of plant meristematic tissues by participating in multiple signaling pathways, thus contributing new insights into the regulatory network involved in plant meristematic tissue development.</div></div>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"806 ","pages":"Article 153412"},"PeriodicalIF":2.2,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146122727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Supernatants from DNase-deficient Prevotella intermedia strains enhance oral squamous cell carcinoma cell migration and invasion by activating inflammatory and epithelial-mesenchymal transition pathways. dna缺陷普雷沃氏菌中间菌株的上清液通过激活炎症和上皮-间质转化途径增强口腔鳞状细胞癌细胞的迁移和侵袭。
IF 2.2 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-05 DOI: 10.1016/j.bbrc.2026.153304
Fumi Seto-Tetsuo, Naoki Katase, Yuko Sasaki, Hideharu Yukitake, Mariko Naito

Introductions: Oral squamous cell carcinoma (OSCC) has been increasingly associated with dysbiosis of the oral microbiome. Among oral pathogens, Prevotella intermedia (P. intermedia) is frequently enriched in patients with OSCC; however, the role of its virulence factors-particularly its deoxyribonuclease (DNase) activity-remains poorly understood.

Methods: We compared the effects of culture supernatants from wild-type P. intermedia OMA14 and DNase-deficient mutant strains (nucA, nucD and nucA nucD) on the migration and invasion of the OSCC cell line SAS, using wound healing and Matrigel invasion assays. Transcriptomic profiling of SAS cells exposed to bacterial supernatants was performed using RNA sequencing (RNA-seq), followed by differential gene expression and pathway enrichment analyses.

Results: Supernatants from the nucA nucD mutant strain significantly enhanced SAS cells migration and invasion compared with those from the OMA14 strain. RNA-seq revealed marked transcriptomic reprogramming, including upregulation of genes related to extracellular matrix degradation, epithelial-mesenchymal transition (EMT), and inflammatory signaling. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses confirmed the enrichment of EMT, cytokine signaling, and tumor-promoting pathways.

Conclusions: Our findings demonstrate a dual role of bacterial DNase activity in SAS cell. Although DNases contribute to immune evasion via neutrophil extracellular trap degradation, their absence enhances tumor invasion by promoting proinflammatory and EMT-related transcriptional programs. These results highlight the complex interplay between microbial nucleases, extracellular DNA, and host signaling, providing novel insights into the contribution of the oral microbiome to OSCC pathogenesis.

{"title":"Supernatants from DNase-deficient Prevotella intermedia strains enhance oral squamous cell carcinoma cell migration and invasion by activating inflammatory and epithelial-mesenchymal transition pathways.","authors":"Fumi Seto-Tetsuo, Naoki Katase, Yuko Sasaki, Hideharu Yukitake, Mariko Naito","doi":"10.1016/j.bbrc.2026.153304","DOIUrl":"https://doi.org/10.1016/j.bbrc.2026.153304","url":null,"abstract":"<p><strong>Introductions: </strong>Oral squamous cell carcinoma (OSCC) has been increasingly associated with dysbiosis of the oral microbiome. Among oral pathogens, Prevotella intermedia (P. intermedia) is frequently enriched in patients with OSCC; however, the role of its virulence factors-particularly its deoxyribonuclease (DNase) activity-remains poorly understood.</p><p><strong>Methods: </strong>We compared the effects of culture supernatants from wild-type P. intermedia OMA14 and DNase-deficient mutant strains (nucA, nucD and nucA nucD) on the migration and invasion of the OSCC cell line SAS, using wound healing and Matrigel invasion assays. Transcriptomic profiling of SAS cells exposed to bacterial supernatants was performed using RNA sequencing (RNA-seq), followed by differential gene expression and pathway enrichment analyses.</p><p><strong>Results: </strong>Supernatants from the nucA nucD mutant strain significantly enhanced SAS cells migration and invasion compared with those from the OMA14 strain. RNA-seq revealed marked transcriptomic reprogramming, including upregulation of genes related to extracellular matrix degradation, epithelial-mesenchymal transition (EMT), and inflammatory signaling. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses confirmed the enrichment of EMT, cytokine signaling, and tumor-promoting pathways.</p><p><strong>Conclusions: </strong>Our findings demonstrate a dual role of bacterial DNase activity in SAS cell. Although DNases contribute to immune evasion via neutrophil extracellular trap degradation, their absence enhances tumor invasion by promoting proinflammatory and EMT-related transcriptional programs. These results highlight the complex interplay between microbial nucleases, extracellular DNA, and host signaling, providing novel insights into the contribution of the oral microbiome to OSCC pathogenesis.</p>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"805 ","pages":"153304"},"PeriodicalIF":2.2,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146131146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corrigendum to "PTX3 activates POSTN and promotes the progression of glioblastoma via the MAPK/ERK signalling axis" [Biochem. Biophys. Res. Commun. 703 (2024) 149665]. “PTX3激活POSTN并通过MAPK/ERK信号轴促进胶质母细胞瘤的进展”[Biochem]的更正。Biophys。《共同法典》,第703(2024)条[149665]。
IF 2.2 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-04 DOI: 10.1016/j.bbrc.2026.153382
Yuhang Wang, Binbin Wang, Wenping Cao, Xiupeng Xu
{"title":"Corrigendum to \"PTX3 activates POSTN and promotes the progression of glioblastoma via the MAPK/ERK signalling axis\" [Biochem. Biophys. Res. Commun. 703 (2024) 149665].","authors":"Yuhang Wang, Binbin Wang, Wenping Cao, Xiupeng Xu","doi":"10.1016/j.bbrc.2026.153382","DOIUrl":"https://doi.org/10.1016/j.bbrc.2026.153382","url":null,"abstract":"","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":" ","pages":"153382"},"PeriodicalIF":2.2,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146123469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Retraction notice to "Carboxyl ester lipase truncation mutant unveils lipotoxicity induced pancreatic β-cell demise" [Biochem. Biophys. Res. Commun. 789 (2025) 152800]. “羧基酯脂肪酶截断突变体揭示脂肪毒性诱导胰腺β细胞死亡”的撤回通知[生物化学]。Biophys。参考文献。789(2025)152800]。
IF 2.2 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-04 DOI: 10.1016/j.bbrc.2026.153368
Jianli Lin, Yi Lin, Jinxin Li, Qinwen Liu, Xiafang Lin, Qinyu Liu, YingHua Luo, Ying Lin, Haohua Chen, Junping Wen
{"title":"Retraction notice to \"Carboxyl ester lipase truncation mutant unveils lipotoxicity induced pancreatic β-cell demise\" [Biochem. Biophys. Res. Commun. 789 (2025) 152800].","authors":"Jianli Lin, Yi Lin, Jinxin Li, Qinwen Liu, Xiafang Lin, Qinyu Liu, YingHua Luo, Ying Lin, Haohua Chen, Junping Wen","doi":"10.1016/j.bbrc.2026.153368","DOIUrl":"10.1016/j.bbrc.2026.153368","url":null,"abstract":"","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":" ","pages":"153368"},"PeriodicalIF":2.2,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146123399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spatial organization of ER-derived protein aggregates in the nucleus requires the Hsp70-family member BiP. er源性蛋白聚集在细胞核中的空间组织需要hsp70家族成员BiP。
IF 2.2 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-04 DOI: 10.1016/j.bbrc.2026.153406
Shoukang Du, Yuhan Wang, Ting Gang Chew

Cells maintain proteostasis by sequestering misfolded proteins into deposition sites. Aggregation-prone endoplasmic reticulum (ER) proteins form membrane-bound nuclear compartments that are cleared during cell division, yet the mechanisms underlying their spatial organization remain unclear. Here, using transcriptomic and proteomic analyses, we identified the ER-localized Hsp70 chaperone BiP as a key player. Genetic depletion or chemical inhibition of BiP prevented nuclear aggregate formation, while manipulating BiP regulators perturbed the aggregate formation. BiP-driven aggregation precedes the inner nuclear membrane synthesis that encapsulated the aggregates. Under proteostatic stress, nuclear aggregates localized adjacent to ER-derived aggregates. Our findings demonstrate that BiP is essential for organizing ER-derived aggregates in the nucleus, which further regulate nuclear proteostasis through spatial interactions with nuclear aggregates.

{"title":"Spatial organization of ER-derived protein aggregates in the nucleus requires the Hsp70-family member BiP.","authors":"Shoukang Du, Yuhan Wang, Ting Gang Chew","doi":"10.1016/j.bbrc.2026.153406","DOIUrl":"https://doi.org/10.1016/j.bbrc.2026.153406","url":null,"abstract":"<p><p>Cells maintain proteostasis by sequestering misfolded proteins into deposition sites. Aggregation-prone endoplasmic reticulum (ER) proteins form membrane-bound nuclear compartments that are cleared during cell division, yet the mechanisms underlying their spatial organization remain unclear. Here, using transcriptomic and proteomic analyses, we identified the ER-localized Hsp70 chaperone BiP as a key player. Genetic depletion or chemical inhibition of BiP prevented nuclear aggregate formation, while manipulating BiP regulators perturbed the aggregate formation. BiP-driven aggregation precedes the inner nuclear membrane synthesis that encapsulated the aggregates. Under proteostatic stress, nuclear aggregates localized adjacent to ER-derived aggregates. Our findings demonstrate that BiP is essential for organizing ER-derived aggregates in the nucleus, which further regulate nuclear proteostasis through spatial interactions with nuclear aggregates.</p>","PeriodicalId":8779,"journal":{"name":"Biochemical and biophysical research communications","volume":"805 ","pages":"153406"},"PeriodicalIF":2.2,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146131150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
RALY promotes Epithelial-mesenchymal transition in Hepatocellular carcinoma by regulating Snail. RALY通过调节Snail促进肝细胞癌上皮-间质转化。
IF 2.2 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2026-02-03 DOI: 10.1016/j.bbrc.2026.153402
Hee-Won Kim, Jungsoo Kim, Jeong-Heon Ko, Jeong Gu Kang

RALY, a heterogeneous nuclear ribonucleoprotein, binds to nascent RNA and participates in multiple aspects of RNA metabolism, including transport, splicing, transcription, and translation. Recent studies have revealed that RALY is overexpressed in various cancers, such as breast, uterine, and liver cancers. This overexpression has been associated with poor patient survival and uncontrolled carcinoma cell proliferation. In this study, we demonstrate that RALY functions as a key regulator of cell proliferation, migration, and invasion in the hepatocellular carcinoma (HCC) cell lines Hep3B and HepG2. Mechanistically, RALY promotes epithelial-mesenchymal transition (EMT) through regulation of the transcription factor Snail. RALY directly binds to Snail mRNA, thereby enhancing its stability. In addition, RALY modulates the TGF-β signaling pathway to promote Snail transcription. Together, our findings establish a functional link between RALY and EMT and reveal a previously unrecognized role of RALY in cancer cell metastasis. Accumulating evidence, including the results presented here, suggests that RALY represents a potential therapeutic target for cancer treatment.

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Biochemical and biophysical research communications
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