Isolation and characterization of the midgut microbiota of Aedes albopictus to identify suitable candidates for paratransgenesis.

IF 3.7 3区 医学 Q1 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH European Journal of Public Health Pub Date : 2025-01-01 DOI:10.1093/eurpub/ckae110
Mersa Darbandsari, Majid Asgari, Mohammad R Abaei, Zahra Ghorbanzadeh, Maryam Derikvand, Patrick Okwarah, Navid Dinparast Djadid, Abbasali Raz
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Abstract

Aedes albopictus is a widely recognized carrier of various pathogens. Its resilient characteristics enable it to easily spread across diverse climates. The microbiota in the midgut of mosquitoes plays a crucial role in the interactions between the host and pathogens and can either enhance or reduce the ability of the insect to transmit diseases. Hence, determining the microorganisms present in the mosquito's digestive system could be a promising approach to developing an effective method of controlling them. Hence, the aim of this study was to investigate the microbial compositions in the midguts of Ae. albopictus mosquitoes collected from the fields of Sistan and Baluchestan Province. The midguts of 60 female mosquitoes were dissected, and their related bacteria were determined using the culture-dependent method. Different colonies were differentiated using the biochemical tests followed by 16S rRNA gene sequencing. The isolated bacteria were identified as belonging to the Asaia, Delftia, Serratia, Aeromonas, Paracoccus, and Planomicrobium genera based on biochemical and molecular analysis. The findings obtained in this study were largely consistent with earlier studies conducted on mosquitoes gathered from different regions throughout the world. Overall, the findings could enhance our understanding of the microbial diversity in Ae. albopictus and aid in the identification of a potent and widespread bacterium for the development of a paratransgenesis tool to combat Aedes-borne infectious diseases.

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白纹伊蚊中肠微生物群的分离和鉴定,以确定合适的准突变候选物。
白纹伊蚊是公认的多种病原体的携带者。它的弹性特性使它能够很容易地在不同的气候中传播。蚊子中肠中的微生物群在宿主和病原体之间的相互作用中起着至关重要的作用,可以增强或降低昆虫传播疾病的能力。因此,确定蚊子消化系统中存在的微生物可能是开发有效控制它们的有效方法的有希望的方法。因此,本研究的目的是研究伊蚊中部肠道的微生物组成。在锡斯坦和俾路支斯坦省田野采集白纹伊蚊。本文解剖了60只雌蚊的中肠,采用培养依赖法对其相关细菌进行了检测。通过生化试验和16S rRNA基因测序对不同菌落进行分化。经生化和分子分析,分离得到的细菌属于Asaia属、Delftia属、Serratia属、Aeromonas属、Paracoccus属和Planomicrobium属。这项研究获得的结果与早期对世界各地不同地区收集的蚊子进行的研究在很大程度上是一致的。总的来说,这些发现可以增强我们对伊蚊微生物多样性的理解。白纹伊蚊,并有助于识别一种有效的和广泛分布的细菌,以开发一种对抗伊蚊传播的传染病的副突变工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
European Journal of Public Health
European Journal of Public Health 医学-公共卫生、环境卫生与职业卫生
CiteScore
5.60
自引率
2.30%
发文量
2039
审稿时长
3-8 weeks
期刊介绍: The European Journal of Public Health (EJPH) is a multidisciplinary journal aimed at attracting contributions from epidemiology, health services research, health economics, social sciences, management sciences, ethics and law, environmental health sciences, and other disciplines of relevance to public health. The journal provides a forum for discussion and debate of current international public health issues, with a focus on the European Region. Bi-monthly issues contain peer-reviewed original articles, editorials, commentaries, book reviews, news, letters to the editor, announcements of events, and various other features.
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