Enzyme cascade nanozyme based colorimetric sensor for detection of uric acid as a biomarker of hyperuricemia

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-01-15 DOI:10.1007/s00604-024-06946-5

Wenrui Ke, Guochen Kuang, Chuangqi Gu, Junhao Wang, Xiao Jiang, Rongjiao Zhu, Haiyuan Wang, Zhen Zhang

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Abstract

A Cr-doped VO₂ nanobelt (Cr/VO₂) with remarkable peroxidase-like activity was synthesized and coupled with uricase to catalyze the cascade reaction for detection of uric acid. Notably, the affinity of Cr/VO₂ for 3,3',5,5'-tetramethylbenzidine dihydrochloride hydrate (TMB) and hydrogen peroxide (H₂O₂) is tenfold and 20-fold higher, respectively, than that of horseradish peroxidase (HRP). The Cr/VO₂ exhibits highly reactive and stable peroxidase activity at temperatures of 20-60 ℃. Thus, the reaction catalyzed by Cr/VO₂ can be carried out at room temperature, greatly simplifying the testing process. This enzyme cascade nanozyme based colorimetric assay enables the selective and simple detection of uric acid within 30 min with a detection limit of only 0.34 μM, and has an observable potential in clinical applications.

Graphical abstract

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.