DNA Methylation of Somatic Tissues in Oysters is Influenced by Sex and Heredity

IF 2.6 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Marine Biotechnology Pub Date : 2025-01-14 DOI:10.1007/s10126-024-10409-6

Wen Teng, Chengxun Xu, Shikai Liu, Hong Yu, Lingfeng Kong, Qi Li

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Abstract

The influence of sex and heredity on DNA methylation in the somatic tissues of mice has been well-documented, with similar hereditary effects reported in honeybees. However, the extent to which these factors affect DNA methylation in molluscan somatic tissues remains poorly understood. In this study, we investigated genomic DNA methylation patterns in the adductor muscle of two genetically distinct oyster strains using whole-genome bisulfite sequencing (WGBS). Our analysis identified significant differences in DNA methylation between sexes, with females exhibiting a global reduction compared to males. Furthermore, approximately half of the differentially methylated sites between the two parental strains were conserved in their offspring. Regions with differential methylation in parents typically exhibited intermediate methylation levels in the F1 progeny, whereas consistently methylated regions in parents maintained similar methylation levels in their progeny. These findings suggest that offspring DNA methylation is strongly influenced by parental methylation profiles, highlighting its potential role in sexual determination in oysters.

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牡蛎体细胞组织DNA甲基化受性别和遗传的影响

性别和遗传对小鼠体细胞组织DNA甲基化的影响已经得到了充分的证明，在蜜蜂中也报道了类似的遗传效应。然而，这些因素对软体动物体细胞组织中DNA甲基化的影响程度仍然知之甚少。在这项研究中，我们使用全基因组亚硫酸盐测序（WGBS）研究了两种遗传上不同的牡蛎菌株内收肌的基因组DNA甲基化模式。我们的分析确定了性别之间DNA甲基化的显著差异，与男性相比，女性表现出整体的减少。此外，两亲本菌株之间大约一半的差异甲基化位点在其后代中保守。双亲中差异甲基化的区域通常在F1后代中表现出中等甲基化水平，而双亲中一致甲基化的区域在其后代中保持相似的甲基化水平。这些发现表明后代DNA甲基化受到亲代甲基化谱的强烈影响，突出了其在牡蛎性别决定中的潜在作用。

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来源期刊

Marine Biotechnology 工程技术-海洋与淡水生物学

CiteScore

4.80

自引率

3.30%

发文量

审稿时长

2 months

期刊介绍： Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.