Aobo Ma , Yiding Zhang , Junduo Chen , Lu Sun , Guang Hong
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引用次数: 0
Abstract
Background/purpose
Titanium dioxide nanotube (TNT) structures have been shown to enhance the early osseointegration of dental implants. Nevertheless, the optimal nanotube diameter for promoting osteogenesis remains unclear due to variations in cell types and manufacture of nanotubes. This study aimed to evaluate the differences in MC3T3-E1 and Saos-2 cells behavior on nanotubes of varying diameters.
Materials and methods
TNT structures were fabricated by anodizing titanium foil at voltages ranging from 15V to 70V and annealed at 450 °C. Surface morphology and wettability were characterized using field emission scanning electron microscopy and water contact angle measurements, respectively. MC3T3-E1 and Saos-2 cells were cultured to evaluate biocompatibility. Early cell morphology and adhesion were visualized by scanning electron microscopy. Cell proliferation was quantified using CCK-8 assays, and differentiation was assessed through alkaline phosphatase assays. Osteogenesis-related gene expression was analyzed by real-time polymerase chain reaction (PCR), measuring runt-related transcription factor 2 (Runx-2), alkaline phosphatase (ALP), collagen type 1 (COL-1), osteocalcin (OCN), and Osteopontin (OPN) gene levels.
Results
Our results found that Saos-2 cells may be more suitable for TNT-related studies compared to MC3T3-E1 cells. Notably, the 65V nanotube group, with a diameter of 135.9 ± 15.83 nm, demonstrated the most significant osteogenic effect in our assays.
Conclusion
We propose that the use and screening of multiple cell lines prior to the evaluation of biomaterials can lead to more accurate in vitro experiments, thereby enhancing the reliability of biomaterial research.
期刊介绍:
he Journal of Dental Sciences (JDS), published quarterly, is the official and open access publication of the Association for Dental Sciences of the Republic of China (ADS-ROC). The precedent journal of the JDS is the Chinese Dental Journal (CDJ) which had already been covered by MEDLINE in 1988. As the CDJ continued to prove its importance in the region, the ADS-ROC decided to move to the international community by publishing an English journal. Hence, the birth of the JDS in 2006. The JDS is indexed in the SCI Expanded since 2008. It is also indexed in Scopus, and EMCare, ScienceDirect, SIIC Data Bases.
The topics covered by the JDS include all fields of basic and clinical dentistry. Some manuscripts focusing on the study of certain endemic diseases such as dental caries and periodontal diseases in particular regions of any country as well as oral pre-cancers, oral cancers, and oral submucous fibrosis related to betel nut chewing habit are also considered for publication. Besides, the JDS also publishes articles about the efficacy of a new treatment modality on oral verrucous hyperplasia or early oral squamous cell carcinoma.