Comparative effects of Serendipita indica and a mix of arbuscular mycorrhizal fungi on the growth, photosynthetic capacity, and proteomics of Schinus terebinthifolius Raddi.

Abstract

Main conclusion: Both, Serendipita indica and AMF, show promise as sustainable biofertilizers for reforestation, improving nutrient uptake and stress tolerance, despite contrasting effects on photosynthetic capacity and biomass allocation. Reclaiming degraded areas is essential for biodiversity conservation and enhancing ecosystem services enhancement, especially when using native species. This study investigated Schinus terebinthifolius Raddi, a native Brazilian species, and its compatibility with plant growth-promoting microorganisms (PGPM), including an endophytic fungus (Serendipita indica) and a consortium of arbuscular mycorrhizal fungi (AMF), to identify effective strategies for reforestation in nutrient-poor environments. We observed growth stimulation by both PGPMs; however, S. indica primarily enhanced root weight, whereas AMF improved shoot weight. S. indica's positive effects on root systems could be attributed to increased auxin levels and altered root architecture, which are critical for seedling establishment in reforestation programs. In terms of nutritional status, both treatments increased the content of most nutrients, with higher micronutrient contents in the shoots and higher macronutrient content in roots of inoculated plants. Despite AMF's role in enhancing photosynthesis, plants inoculated with these fungi showed reduced photosynthetic capacity traits, possibly due to lower leaf nitrogen content. The proteomic analysis of Schinus terebinthifolius leaf extracts revealed that, despite the upregulation of several proteins associated with the photosynthetic apparatus in response to S. indica treatment, no enhancement in photosynthetic capacity was observed. We also found several proteins related to oxidative stress in plants inoculated with both fungi, indicating a greater tolerance to adverse environmental conditions. These findings underscore the potential of both, S. indica and AMF, as sustainable alternatives to chemical fertilizers in reforestation efforts, enhancing seedling quality and survival in nutrient-poor soils.