Gut microbial communities and transcriptional profiles of black soldier fly (Hermitia illucens) larvae fed on fermented sericulture waste

IF 7.1 2区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL Waste management Pub Date : 2025-01-12 DOI:10.1016/j.wasman.2025.01.011

Fareed Uddin Memon , Yanqing Zhu , Ying Cui , Xingbao Feng , Sheraz Ahmad , Peng Zeng , Farhan Nabi , Dengjian Hao , Zhijun Huang , Gianluca Tettamanti , Ling Tian

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Abstract

Sericulture waste poses significant challenges to industrial and environmental safety. Black soldier fly larvae (BSFL) offer a promising solution for organic waste management by converting it into insect protein. This study aimed to develop a microbial fermented method for utilizing sericulture waste to feed BSFL and explore the underlying mechanisms. Our results showed that all fermented sericulture waste groups had positive effects on body weight, survival rate, substrate consumption rate, and substrate conversion rate. Metagenomic analysis revealed a notable increase in the abundances of commensal genera, including Sedimentibacter, Clostridium, Enterococcus, Bacteroides, and Bacillus, in the gut of BSFL fed on sericulture waste fermented with the most effective combination of microbial strains (B. subtilis, B. licheniformis, and E. faecalis). In contrast, BSFL reared on unfermented sericulture waste exhibited higher abundances of potentially pathogenic and harmful genera, including Providencia, Klebsiella, Escherichia, Brucella, and Enterobacter. Clusters of orthologous genes (COG) analysis indicated that altered microbial communities in the fermented group mainly participated in metabolic pathways, defense mechanism, and signal transduction mechanism. Transcriptome analysis further revealed that the upregulated genes were functionally associated with key metabolic pathways and immune mechanisms in the fermented group. These findings underscore the pivotal role of selected microbial fermentation in utilizing sericulture waste as BSFL feed, providing a sustainable solution for organic waste management.

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以发酵养蚕废弃物为食的黑实蝇（Hermitia illucens）幼虫的肠道微生物群落和转录谱图。

蚕桑废弃物对工业和环境安全构成重大挑战。黑兵蝇幼虫（BSFL）通过将有机废物转化为昆虫蛋白，为有机废物管理提供了一种很有前途的解决方案。本研究旨在建立一种利用蚕桑废弃物进行微生物发酵的方法，并探讨其作用机制。结果表明，各发酵蚕桑废弃物处理组对肉鸡体重、成活率、底物消耗率和底物转化率均有显著影响。元基因组分析显示，以最有效的微生物菌株（枯草芽孢杆菌、地衣芽孢杆菌和粪芽孢杆菌）组合发酵的蚕桑废弃物为食的BSFL肠道中共生菌属的丰度显著增加，包括沉积杆菌、梭状芽孢杆菌、肠球菌、拟杆菌和芽孢杆菌。相比之下，在未发酵的蚕桑废物上饲养的BSFL显示出更高的潜在致病性和有害属，包括普罗维登氏菌、克雷伯氏菌、埃希氏菌、布鲁氏菌和肠杆菌。同源基因簇（Clusters of orthologous genes， COG）分析表明，发酵组中改变的微生物群落主要参与了代谢途径、防御机制和信号转导机制。转录组分析进一步揭示，在发酵组中，上调的基因在功能上与关键的代谢途径和免疫机制相关。这些发现强调了选择微生物发酵在利用蚕桑废物作为BSFL饲料中的关键作用，为有机废物管理提供了可持续的解决方案。

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来源期刊

Waste management 环境科学-工程：环境

CiteScore

15.60

自引率

6.20%

发文量

492

审稿时长

39 days

期刊介绍： Waste Management is devoted to the presentation and discussion of information on solid wastes,it covers the entire lifecycle of solid. wastes. Scope: Addresses solid wastes in both industrialized and economically developing countries Covers various types of solid wastes, including: Municipal (e.g., residential, institutional, commercial, light industrial) Agricultural Special (e.g., C and D, healthcare, household hazardous wastes, sewage sludge)