Long-term response mechanism of bacterial communities to chemical oxidation remediation in petroleum hydrocarbon contaminated groundwater

Abstract

The limited understanding of microbial response mechanism remains as a bottleneck to evaluate the long-term remediation effectiveness of in situ chemical oxidation in contaminated groundwater. In this study, we investigated long-term response of bacterial communities throughout five remediation stages of pre-oxidation, early-oxidation, late-oxidation, early-recovery and late-recovery. By analyzing bacterial biomass, taxa, diversity and metabolic functions, this work identified the consistently suppressed glyceraldehyde-3-phosphate dehydrogenase pathway and the enrichment of naphthalene degradation pathways for secondary products, suggesting persistent oxidation stress and enhanced microbial utilization of lower-molecular weight carbon sources at the oxidation and early-recovery stages. The dominant microbial clusters shifted from r-strategists to K-strategists and then back to r-strategists, indicating their higher degradation efficiency of petroleum hydrocarbons throughout the oxidation process. The changes in stability and stochastic assembly of bacterial communities during in situ chemical oxidation suggested that oxidative stress, carbon source addition and carbon source limitation as the main influential factors of bacterial community succession at the oxidation, early-recovery and late-recovery stage, respectively. Our findings highlighted the complex recovery and underlying mechanisms of groundwater bacterial communities during in situ chemical oxidation process, and provided valuable insights for effective and long-term site management after in situ chemical oxidation practices.