Elucidating cardiomyocyte heterogeneity and maturation dynamics through integrated single-cell and spatial transcriptomics

IF 4.1 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES iScience Pub Date : 2025-01-17 Epub Date: 2024-12-13 DOI:10.1016/j.isci.2024.111596

Xiaoying Wang , Lizhi Cao , Rui Chang , Junwei Shen , Linlin Ma , Yanfei Li

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Abstract

The intricate development and functionality of the mammalian heart are influenced by the heterogeneous nature of cardiomyocytes (CMs). In this study, single-cell and spatial transcriptomics were utilized to analyze cells from neonatal mouse hearts, resulting in a comprehensive atlas delineating the transcriptional profiles of distinct CM subsets. A continuum of maturation states was elucidated, emphasizing a progressive developmental trajectory rather than discrete stages. This approach enabled the mapping of these states across various cardiac regions, illuminating the spatial organization of CM development and the influence of the cellular microenvironment. Notably, a subset of transitional CMs was identified, characterized by a transcriptional signature marking a pivotal maturation phase, presenting a promising target for therapeutic strategies aimed at enhancing cardiac regeneration. This atlas not only elucidates fundamental aspects of cardiac development but also serves as a valuable resource for advancing research into cardiac physiology and pathology, with significant implications for regenerative medicine.

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通过整合单细胞和空间转录组学阐明心肌细胞异质性和成熟动力学。

哺乳动物心脏的复杂发育和功能受到心肌细胞（CMs）异质性的影响。在这项研究中，单细胞和空间转录组学被用于分析新生小鼠心脏细胞，从而形成了一个全面的图谱，描绘了不同CM亚群的转录谱。一个连续的成熟状态被阐明，强调一个渐进的发展轨迹，而不是离散的阶段。这种方法能够在不同的心脏区域绘制这些状态，阐明CM发展的空间组织和细胞微环境的影响。值得注意的是，过渡性CMs的一个子集被鉴定出来，其特征是一个关键成熟阶段的转录特征，为旨在增强心脏再生的治疗策略提供了一个有希望的靶点。该图谱不仅阐明了心脏发育的基本方面，而且为推进心脏生理学和病理学研究提供了宝贵的资源，对再生医学具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

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