Toward a phylogenomic classification of magnoliids.

Abstract

Premise: Magnoliids are a strongly supported clade of angiosperms. Previous phylogenetic studies based primarily on analyses of a limited number of mostly plastid markers have led to the current classification of magnoliids into four orders and 18 families. However, uncertainty remains regarding the placement of several families.

Methods: For the first comprehensive phylogenomic analysis of magnoliids as a whole, we sampled 235 species from 199 (74%) genera and representing all families and most subfamilies and tribes. We analyzed newly generated data from the Angiosperms353 probe set using both coalescent and concatenation analyses and testing the impact of multiple filtering and alignment strategies.

Results: While our results generally provide further support for previously established phylogenetic relationships in both magnoliids as a whole and large families including Annonaceae and Lauraceae, they also provide new evidence for previously ambiguous relationships. In particular, we found support for the position of Hydnoraceae as sister to the remainder of Piperales, though evidence was conflicting, and resolved the backbone of relationships among most genera of Myristicaceae. Different analytical strategies tended to have rather small effects on branch support and topology.

Conclusions: Although some of our results are limited by low gene recovery for a number of taxa and significant gene tree conflict for some relationships, this study represents a significant step toward reconstructing the evolutionary history of a major lineage of angiosperms. Based on these results, we present an updated phylogenetic classification for magnoliids, recognizing 21 families, summarizing previously established subfamilies and tribes, and describing new tribes for Myristicaceae.