American Journal of Botany最新文献

Premise: The warmer and drier atmospheric conditions of urban environments challenge plant performance to different extents based on a species' ability to acclimate to the conditions. We evaluated the influence of species origin and thermal niche on the acclimation of leaf traits and shifts in the occupation of the functional trait space of 10 tree species growing in two environmentally contrasting sites in Bogotá, Colombia.

Methods: We measured six leaf traits per species in both sites and used generalized linear models to evaluate the influence of origin and thermal niche on acclimation of leaf traits and t-tests to analyze shifts in the occupation of the functional trait space.

Results: Species origin predicted thermal tolerance and morphological trait acclimation to warmer conditions. Although exotic species decreased thermal tolerance at the warmer site, species from both origins acclimated traits consistently. Shifts in the occupation of the functional trait space varied between origins; warmer conditions reduced the size of the functional trait space of exotics and increased the phenotypic similarity of natives. Thermal tolerance acclimation and changes in functional trait space varied across species. Finally, thermal niche metrics were uncoupled from species origin and failed to explain the acclimation capacity of the studied species.

Conclusions: Although species origin influenced acclimation to warmer conditions, the effect of origin was not related to species' thermal niches. Our results provide crucial information for decision-makers involved in designing urban and peri-urban green spaces that can withstand climate change.

Premise: The ability of plants to adapt or acclimate to climate change is inherently linked to their interactions with symbiotic microbes, notably fungi. However, it is unclear whether fungal symbionts from different climates have different impacts on the outcome of plant-fungal interactions, especially under environmental stress.

Methods: We tested three provenances of fungal inoculum (originating from dry, moderate or wet environments) with one host plant genotype exposed to three soil moisture regimes (low, moderate and high). Inoculated and uninoculated plants were grown in controlled conditions for 151 days, then shoot and root biomass were weighed and fungal diversity and community composition determined via amplicon sequencing.

Results: The source of inoculum and water regime elicited significant changes in plant resource allocation to shoots versus roots, but only specific inocula affected total plant biomass. Shoot biomass increased in the high water treatment but was negatively impacted by all inoculum treatments relative to the controls. The opposite was true for roots, where the low water treatment led to greater proportional root biomass, and plants inoculated with wet site fungi allocated significantly more resources to root growth than dry- or moderate-site inoculated plants and the controls. Fungal communities of shoots and roots partitioned by inoculum source, water treatment, and the interaction of the two.

Conclusions: The provenance of fungi can significantly affect total plant biomass and resource allocation above- and belowground, with fungi derived from more extreme environments eliciting the strongest plant responses.

Premise: Considering rapidly changing fire regimes due to anthropogenic disturbances to climate and fuel loads, it is crucial to understand the underpinnings driving fire-adapted trait evolution. Among the oldest lineages affected by fire is Coniferae. This lineage occupies a variety of fire prone and non-fire prone habitats across all hemispheres and has four fire-adapted traits: (1) thick bark; (2) serotiny; (3) seedling grass stage; and (4) resprouting ability. We seek to determine the historic origins of these traits, the degree of convergent evolution among species, how fire adaptations affect diversification rates in conifers, and if there is a link between climate and the evolution of fire adaptations.

Methods: To investigate these questions, we use a combination of ancestral state reconstructions, multiple diversification analyses, and Pagel trait correlations.

Results: Our results point to multiple evolutionary origins of fire adaptations. We find certain climates, particularly Subtropical and Mediterranean, are highly correlated with species possessing fire adaptations. Several lineages evolved fire adaptations after the Mid-Miocene Climactic Optimum, which coincides with the expansion of the then novel Mediterranean Climate type. Generally possessing a fire adaptation does not increase diversification rates, with the possible exceptions of Pinus subsections Australes and Ponderosae.

Conclusions: The appearance of novel climates and associated fire regimes seem to have been the primary drivers of fire adaptation evolution in conifers. However, most increases in diversification rates are within clades that responded favorably to cooler drier climates post Mid-Miocene Climactic Optimum, regardless of whether the clade is fire adapted.

Premise: Primroses famously employ a system that simultaneously expresses distyly and filters out self-pollen. Other species in the Primulaceae family, including Lysimachia monelli (blue pimpernel), also express self-incompatibility (SI), but involving a system with distinct features and an unknown molecular genetic basis.

Methods: We utilize a candidate-based transcriptome sequencing (RNA-seq) approach, relying on candidate T2/S-RNase Class III and S-linked F-box-motif-containing genes and harnessing the unusual evolutionary and genetic features of SI, to examine whether an RNase-based mechanism underlies SI in L. monelli. We term this approach "SI detection with RNA-seq" (SIDR).

Results: The results of sequencing, crossing, population genetics, and molecular evolutionary features each support a causal association linking the recovered genotypes with SI phenotypes. The finding of RNase-based SI in Primulaceae (Ericales) all but cements the long-held view that this mechanism was present in the ancestral pentapetal eudicot, whose descendants now comprise two-thirds of angiosperms. It also significantly narrows the plausible maximum age for the heterostyly evolution within the family.

Conclusions: SIDR is powerful, flexible, inexpensive, and most critically enables work in often-neglected species. It may be used with or without candidate genes to close enormous gaps in understanding the genetic basis of SI and the history of breeding system evolution.

Premise: Understanding how population dynamics vary in space and time is critical for understanding the basic life history and conservation needs of a species, especially for narrow endemic species whose populations are often in similar environments and therefore at increased risk of extinction under climate change. Here, we investigated the spatial and temporal variation in population dynamics of Ranunculus austro-oreganus, a perennial buttercup endemic to fragmented prairie habitat in one county in southern Oregon.

Methods: We performed demographic surveys of three populations of R. austro-oreganus over 4 years (2015-2018). We used size-structured population models and life table response experiments to investigate vital rates driving spatiotemporal variation in population growth.

Results: Overall, R. austro-oreganus had positive or stable stochastic population growth rates, though individual vital rates and overall population growth varied substantially among sites and years. All populations had their greatest growth in the same year, suggesting potential synchrony associated with climate conditions. Differences in survival contributed most to spatial variation in population growth, while differences in reproduction contributed most to temporal variation in population growth.

Conclusions: Populations of this extremely narrow endemic appear stable, with positive growth during our study window. These results suggest that populations of R. austro-oreganus are able to persist if their habitat is not eliminated by land-use change. Nonetheless, its narrow distribution and synchronous population dynamics suggest the need for continued monitoring, particularly with ongoing habitat loss and climate change.

Premise: Tree structure and function are constrained by and acclimate to climatic conditions. Drought limits plant growth and carbon acquisition and can result in "legacy" effects that last beyond the period of water stress. Leaf and twig-level legacy effects of past water abundance, such as that experienced by trees that established under wetter conditions are unknown.

Methods: In an 18-year forest irrigation experiment, we explored the lasting structural impact of past water richness on leaves and twigs of Pinus sylvestris using synchrotron-based X-ray microscopy. We compared 47 anatomical traits among never-irrigated control trees, trees irrigated for 18 years, and formerly irrigated trees, 7 years after their return to control-level water availability in this naturally dry forest.

Results: We found that legacy effects induced by a period of experimental irrigation continue to shape the structure of new leaves and twigs long after a sharp decrease in water availability. Legacy effects shaping twigs were present but dissipating, while leaf traits displayed long-lasting effects on structural strategy, with extreme values for traits associated with high water stress and low productivity.

Conclusions: Mature trees acclimating to an increasingly dry world may be at a disadvantage compared to young trees that have known only chronic drought. However, these young trees may be capable of thriving in sites of drought-related forest decline especially if planted while larger individuals are still present to support tree establishment. Without a legacy of past water abundance, trees in future forests may be better equipped to cope with our rapidly changing climate.

Premise: Magnoliids are a strongly supported clade of angiosperms. Previous phylogenetic studies based primarily on analyses of a limited number of mostly plastid markers have led to the current classification of magnoliids into four orders and 18 families. However, uncertainty remains regarding the placement of several families.

Methods: For the first comprehensive phylogenomic analysis of magnoliids as a whole, we sampled 235 species from 199 (74%) genera and representing all families and most subfamilies and tribes. We analyzed newly generated data from the Angiosperms353 probe set using both coalescent and concatenation analyses and testing the impact of multiple filtering and alignment strategies.

Results: While our results generally provide further support for previously established phylogenetic relationships in both magnoliids as a whole and large families including Annonaceae and Lauraceae, they also provide new evidence for previously ambiguous relationships. In particular, we found support for the position of Hydnoraceae as sister to the remainder of Piperales, though evidence was conflicting, and resolved the backbone of relationships among most genera of Myristicaceae. Different analytical strategies tended to have rather small effects on branch support and topology.

Conclusions: Although some of our results are limited by low gene recovery for a number of taxa and significant gene tree conflict for some relationships, this study represents a significant step toward reconstructing the evolutionary history of a major lineage of angiosperms. Based on these results, we present an updated phylogenetic classification for magnoliids, recognizing 21 families, summarizing previously established subfamilies and tribes, and describing new tribes for Myristicaceae.

Premise: Despite the high functional importance of endophytes, we still have limited understanding of the biotic and abiotic factors that influence colonization of plant hosts along major ecological gradients and lack quantitative estimates of their colonization extent. In this study, we hypothesized that the developmental stage of the ecosystem will affect the levels of bacterial and fungal endophytic assemblages in the foliar endosphere.

Methods: We quantified levels of bacterial and fungal endophytes in leaves of four plant hosts at four stages of vegetation succession using an optimized qPCR protocol with bacteria-specific 16S and fungi-targeting primers.

Results: (1) The ecosystem developmental stage did not have a significant effect on the colonization levels of bacterial or fungal endophytes. (2) Colonization levels by bacterial and fungal endophytes were governed by different mechanisms. (3) Endophytic colonization levels and their relationship to foliar tissue stoichiometry were highly host specific.

Conclusions: Quantifying colonization levels is important in the study of endophytic ecology, and the fast, relatively low-cost qPCR-based method can supply useful ecological information, which can significantly enhance the interpretation potential of descriptive data generated, for example, by next-generation sequencing.

Premise: The movement of lineages into novel areas can promote ecological opportunity and adaptive radiation, leading to significant species diversity. Not all studies, however, have identified support for ecological opportunity associated with novel intercontinental colonizations. To gain key insights into the drivers of ecological opportunity, we tested whether intercontinental dispersals resulted in ecological opportunity using the Hydrangeaceae-Loasaceae clade, which has numerous centers of diversity across the globe.

Methods: A time-calibrated phylogeny was reconstructed from four molecular markers. We tested for bursts of speciation rates followed by a decrease as expected phylogenetic patterns under an ecological opportunity model. Ancestral ranges were estimated using historical biogeographic analyses to examine the relationships of ancestral distributions and habitats with speciation and extinction rates.

Results: Hydrangeaceae and Loasaceae originated in arid Mesoamerica, then dispersed into South America, Eurasia, and eastern North America. Six clades experienced increased diversification rates, but those increases were not associated with transitions into new continental areas. Mentzelia section Bartonia was the only clade that exhibited a burst of speciation followed by a decrease. Both families originated in arid environments and experienced multiple transitions into mesic and tropical environments, but Loasaceae experienced a higher speciation-to-extinction ratio than Hydrangeaceae in the western Nearctic.

Conclusions: Dispersal between continents did not trigger speciation rate shifts in Loasaceae and Hydrangeaceae. Instead, shifts occurred in regions inhabited by intrafamilial relatives and were likely driven by climate change in the Miocene, where species in drier microhabitats diversified into newly created habitats.

Premise: Prairies are among the most threatened biomes due to changing patterns of climate and land use, yet information on genetic variation in key species that would inform conservation is often limited. We assessed evidence for the geographic scale of population-level variation in growth of two species of prairie clover and of their symbiotic associations with nitrogen-fixing bacteria.

Methods: Seed representing two species, Dalea candida and D. purpurea, from the same five source populations were planted into an experimental site in Minnesota. We assessed variation within and among source populations in plant growth and in numbers of nodules and evaluated the relationship of growth and nodulation levels.

Results: Plant growth varied among source populations, with greater differences among populations of D. purpurea than of D. candida. We did not detect a relationship between plant growth and distance of source populations from the experimental site. Populations of both species were equally likely to develop nodules at the experimental site, but the numbers of nodules were lowest for the most distantly sourced populations. Plant growth was positively correlated with the number of nodules, and this relationship varied considerably within and among populations.

Conclusions: Environmental heterogeneity at local and regional scales maintains substantial levels of genetic variation in plant populations within remnant prairie preserves. Further, association with rhizobia at a restoration site can improve growth of widely sourced plant populations. The in situ maintenance of plant genetic variation and species diversity provides resources for conservation and maintenance of prairie biomes.