Multipurpose triadic MXene/garlic/gellan gum-based architecture in the horizon of bone tissue regeneration

Abstract

The use of bioresorbable compositions has been considered a promising therapeutic approach for treating compromised bone tissues. Gellan gum (GG) is a predominant polysaccharide recognized for its exceptional biocompatibility and biodegradability, facile bio-fabrication, and customizable mechanical attributes, rendering it well-suited for developing versatile bone scaffolds. On the other hand, MXene nanosheets have been declared a representational filler to augment the osteogenic effect and amend the mechanical properties of the polymeric biomaterials. Herein, the GG/MXene system was formulated to investigate the synergistic impact of gellan gum and MXene on promoting bone tissue engineering. Accordingly, Ti₃C₂T_x MXene nanogalleries were synthesized and loaded with 1, 3, and 5 wt% ratios into the GG matrix to fortify the overall performances. Based on the outcomes, the GG containing 1 wt% MXene showed a homogeneous surface with an optimized topography, providing greater amorphous regions (15%), boosted hydrophilicity (27.5°), and a favorable Young's modulus (13.43 MPa). Additionally, the designed scaffold provided exceptional osteogenetic adhesion and bactericidal behavior against both Gram-positive (S. aureus) and -negative (E. coli) bacteria. To achieve more desirable biological performance, 1 ml garlic extract (GA) was introduced to the freeze-dried composite network. The results exhibited better cell attachment in the porous GA-mediated scaffold with furthered antibacterial features through an increase in the zone diameter breakpoint from 4.8 ± 0.2 and 5.0 ± 0.1 mm to 5.9 ± 0.3 and 6.2 ± 0.2 mm against S. aureus and E. coli, respectively. Therefore, embedding GA, alongside MXene layered nanomaterials, into the GG-based matrix could provide a convenient scaffolding architecture for guided bone regeneration, facilitating appropriate cell attachment, growth, and proliferation.