Effects of litter and root inputs on soil microbial community structure in subtropical natural and plantation forests

IF 3.9 2区农林科学 Q1 AGRONOMY Plant and Soil Pub Date : 2025-01-18 DOI:10.1007/s11104-025-07218-8

Cuijuan Wang, Weisheng Lin, Shuxian Jia, Shidong Chen, Decheng Xiong, Chao Xu, Zhijie Yang, Xiaofei Liu, Yusheng Yang

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Abstract

Aims

Soil microorganisms play a pivotal role in forest ecosystems geochemical cycle, yet their responses to variations in aboveground and belowground carbon inputs remain insufficiently understood. Variations in plant-derived carbon inputs are expected to influence microbial community structures. This study aims to investigate how changes in these carbon inputs affect the composition and diversity of soil microbial communities across different forest ecosystems in the subtropics forest.

Methods

Natural forest and plantation of Castanopsis carlesii in the subtropics were selected, with three 20 m × 20 m plots established in each forest. Six treatments were applied in a randomized complete block design, with aboveground litter excluded using nylon nets and root exclusion achieved by trenching. Soil microbial community structure under each treatment was analyzed using high-throughput amplicon sequencing.

Results

Litter and root treatments significantly altered microbial community composition and diversity. Bacterial communities, dominated by Acidobacteria, Proteobacteria, and Actinobacteria, showed no significant structural changes in response to treatments but were influenced by forest type. In contrast, fungal communities, dominated by Basidiomycota and Ascomycota, exhibited reduced diversity under root exclusion and litter treatments in natural forests. Soil properties such as moisture, temperature, DOC, and DON were key drivers of microbial community structure and diversity.

Conclusion

These results underscore the role of ecological niche differences between forest forests and alterations in soil chemical properties induced by litter and root inputs in shaping soil microbial communities. These findings offer insights into the mechanisms governing nutrient cycling and soil carbon dynamics in subtropical forest ecosystems.

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凋落物和根系输入对亚热带天然林和人工林土壤微生物群落结构的影响

目的土壤微生物在森林生态系统地球化学循环中发挥着关键作用，但其对地上和地下碳输入变化的响应尚不清楚。植物源碳输入的变化预计会影响微生物群落结构。本研究旨在探讨这些碳输入的变化如何影响亚热带森林不同森林生态系统土壤微生物群落的组成和多样性。方法选择亚热带地区的栲人工林和天然林，每个人工林建立3个20 m × 20 m的样地。采用随机完全区组设计，采用尼龙网排除地上凋落物，采用挖沟法排除根系。采用高通量扩增子测序技术分析各处理下土壤微生物群落结构。结果凋落物和根系处理显著改变了微生物群落组成和多样性。细菌群落以酸杆菌、变形杆菌和放线菌为主，在不同处理条件下结构变化不明显，但受森林类型的影响。而天然林真菌群落多样性在排根和凋落物处理下呈下降趋势，主要以担子菌门和子囊菌门为主。水分、温度、DOC和DON等土壤特性是微生物群落结构和多样性的关键驱动因素。结论森林生态位差异、凋落物和根系输入引起的土壤化学性质变化对土壤微生物群落的形成具有重要影响。这些发现为亚热带森林生态系统养分循环和土壤碳动态的调控机制提供了新的思路。

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来源期刊

Plant and Soil 农林科学-农艺学

CiteScore

8.20

自引率

8.20%

发文量

543

审稿时长

2.5 months

期刊介绍： Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.