Immobilization of ammonia-oxidizing bacteria using mycelial pellets: Preparation, characteristics, and application for nitritation

IF 9 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING Bioresource Technology Pub Date : 2025-01-15 DOI:10.1016/j.biortech.2025.132083

Ming Chen , Lei Jin , Xiaoying Liu , Renjie Li , Huiling Xian , Chao Guo

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Abstract

Ammonia-oxidizing bacteria (AOB) sourced from an aerobic granular sludge (AGS) process were rapidly enriched by progressively increasing ammonia nitrogen (NH₄⁺-N) loads, achieving a Nitrosomonas abundance of 20.7 % and a nitrite accumulation rate exceeding 80 %. Mycelial pellets formed by Cladosporium, isolated from the same AGS system, provided a porous surface structure for the immobilization of the enriched AOB, creating mycelial pellet/AOB composites. Robust microbial colonization and aggregation in mycelial pellet porous matrix were facilitated by a higher level of extracellular polymeric substances (EPS) compared to conventional AGS. Static tests showed a maximum NH₄⁺-N oxidation rate of 17.7 mg/(gMLVSS·h), higher than free AOB (8.5 mg/(gMLVSS·h)). In multi-recycling tests, the composites maintained 96.6 % NH₄⁺-N oxidation, demonstrating superior repeatability and stability. The results highlight advantages of mycelial pellets as biocompatible carriers in immobilizing AOB sourced from the same system, offering insights into improved nitritation performance and durability, making them promising for practical wastewater treatment.

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用菌丝球固定化氨氧化细菌：制备、特性及在硝化中的应用

来自好氧颗粒污泥（AGS）工艺的氨氧化细菌（AOB）通过逐步增加氨氮（NH4+-N）负荷而迅速富集，亚硝酸单胞菌丰度达到20.7%，亚硝酸盐积累率超过80%。由枝孢菌形成的菌丝球，从相同的AGS体系中分离出来，为固定富集的AOB提供了多孔的表面结构，形成菌丝球/AOB复合材料。与常规AGS相比，细胞外聚合物（EPS）水平的提高促进了菌丝球多孔基质中微生物的定植和聚集。静态试验结果表明，NH4+-N氧化速率最高可达17.7 mg/(gMLVSS·h)，高于游离AOB （8.5 mg/(gMLVSS·h)）。在多次循环试验中，复合材料的NH4+-N氧化率保持在96.6%，具有良好的重复性和稳定性。结果强调了菌丝球作为生物相容性载体在固定来自同一系统的AOB方面的优势，为改善硝化性能和耐久性提供了见解，使其在实际废水处理中具有前景。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.