Biodegradation of polyethylene with polyethylene-group-degrading enzyme delivered by the engineered Bacillus velezensis

Abstract

Microplastics (MPs) pose an emerging threat to vegetable growing soils in Harbin, which have a relatively high abundance (11,065 n/kg) with 17.26 of potential ecological risk of single polymer hazard (EI) and 33.92 of potential ecological risk index (PERI). Polyethylene (PE) is the main type of microplastic pollution in vegetable growing soils in Harbin. In this study, the engineered Bacillus velezensis with polyethylene-group-degrading enzyme pathway (BCAv-PEase) was constructed to enhance the degradation of MPs of PE (PE-MPs). BCAv-PEase increased the biodegradation of PE-MPs, promoted weight loss of PE films, elevated surface tension, and decreased the surface hydrophobicity of PE through upregulating activities of depolymerases, dehydrogenase, and catalase. Mechanism analysis showed that BCAv-PEase degraded PE-MPs by promoting the secretion of PEase, thereby leading to the generation of new oxygenated functional groups within the PE-MPs substrate, which further accelerated the metabolic pathway of PE-MPs. The analysis of the microbial community during the PE-MPs degradation processes revealed that BCAv-PEase emerged as the principal bacterial player and stimulated the abundance of microbes and functional genes associated with the biodegradation of PE. In conclusion, this study provides a potential mechanism for biodegradation of PE-MPs mediated by BCAv-PEase via modulating substrate selectivity and optimizing biocatalytic pathways.