First complete genome sequence of tulip mild mottle mosaic virus (Ophiovirus tulipae)

IF 2.5 4区医学 Q3 VIROLOGY Archives of Virology Pub Date : 2025-01-17 DOI:10.1007/s00705-025-06224-6

Yutaro Neriya, Kakeru Hamamoto, Tominari Kobayashi, Shunsuke Nakase, Rena Kurosawa, Tomohiro Suzuki, Hisashi Nishigawa, Toshiyuki Morikawa, Tomohide Natsuaki

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Abstract

Tulip mild mottle mosaic disease, caused by tulip mild mottle mosaic virus (TMMMV, species Ophiovirus tulipae), was first reported in Japan in 1979. TMMMV has a negative-sense ssRNA genome and is closely related to ophioviruses such as Mirafiori lettuce big vein virus (MLBVV, Ophiovirus mirafioriense). However, its complete nucleotide sequence has not yet been reported. Therefore, in this study, we determined the complete nucleotide sequences of all four TMMMV segments. The 5′-terminal tetranucleotide sequences were highly conserved, and the five bases at the 5′ end were identical in all segments. Nucleotide sequence comparisons of the four segments showed the highest sequence identity to those of MLBVV (70–76% for the genomic RNA sequences and 81–87% for the amino acid sequences of each ORF, except for ORF2). The amino acid sequences of the coat proteins of TMMMV and MLBVV were found to be 83.1% identical, which is below the 85% threshold for assignment to the same species, indicating that these viruses belong to different species in the same genus.

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郁金香轻度斑驳花叶病毒（ophivirus tulipae）首个全基因组序列

郁金香轻度斑驳花叶病是由郁金香轻度斑驳花叶病毒（TMMMV）引起的，于1979年在日本首次报道。TMMMV具有负义ssRNA基因组，与Mirafiori lettuce big vein virus （MLBVV, ophivirus mirafioriense）等蛇毒病毒密切相关。但其完整的核苷酸序列尚未见报道。因此，在本研究中，我们确定了所有四个TMMMV片段的完整核苷酸序列。5′端四核苷酸序列高度保守，5′端的5个碱基在所有片段中完全相同。四个片段的核苷酸序列比较表明，与MLBVV的序列一致性最高（基因组RNA序列为70-76%，氨基酸序列为81-87%，ORF2除外）。TMMMV和MLBVV的外壳蛋白氨基酸序列相同度为83.1%，低于同一种的85%阈值，表明这两种病毒属于同一属的不同种。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.